CORE--TRANSGENIC AND CHIMERIC MOUSE FACILITY

核心——转基因和嵌合小鼠设施

• 批准号: 6989546
• 负责人: RONALD B HERBERMAN
• 金额: $ 3.66万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-22 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989546
• 关键词: animal colony; biomedical facility; cryopreservation; electroporation; gene targeting; genetic manipulation; genetically modified animals; laboratory mouse; microinjections; nuclear transfer; tissue mosaicism

The primary goal of the newly proposed Transgenic and Chimeric Mouse Facility (TCMF) is to provide a reliable service to members of the University of Pittsburgh Cancer Institute (UPCI) and to others in the University of Pittsburgh scientific community. This service includes the generation of new transgenic and knockout mouse lines, derivation of existing lines via transfer of pre-implantation embryos, cryopreservation of mouse embryos. The TCMF uses state-of-the-art technologies and has staff with the technical expertise to provide these services. Another important aim of the TCMF is to provide consultations with UPCI investigators to assist in the design of experiments to modify the genetic composition of mice. These experiments are primarily transgenic and targeted mutagenesis experiments (knockouts and knockins). The consultations focus on the efficient design of DNA plasmids, containing transgene and gene targeting constructs, to ensure the success of an investigator's in vivo mouse experiments. As part of this assistance, the TCMF can provide a variety of different plasmids to ensure the accuracy of the DNA constructs. Finally, the TCMF will provide an education and training service to investigators wishing to learn and incorporate transgenic and gene targeting technologies into their own laboratories. Technologies provided There are five specific technical services that the TCMF provides to investigators: Pronuclear microinjection (PNM) ES cell electroporation (ESE) ES cell injection (ESM) Line derivation (DER) Embryo cryopreservation (CRY)

新提议的转基因和嵌合小鼠设施（TCMF）的主要目标是为匹兹堡大学癌症研究所（UPCI）的成员和匹兹堡大学科学界的其他人提供可靠的服务。这项服务包括新的转基因和基因敲除小鼠品系的产生，通过移植植入前胚胎对现有品系的衍生，小鼠胚胎的冷冻保存。TCMF使用最先进的技术，并拥有具备技术专长的工作人员来提供这些服务。 TCMF的另一个重要目的是为UPCI研究人员提供咨询，以帮助设计实验来修改小鼠的遗传组成。这些实验主要是转基因和靶向诱变实验（敲除和敲入）。磋商的重点是DNA质粒的有效设计，含有转基因和基因靶向结构，以确保研究人员的体内小鼠实验的成功。作为这种辅助的一部分，TCMF可以提供各种不同的质粒以确保DNA构建体的准确性。 最后，TCMF将为希望学习转基因和基因靶向技术并将其纳入自己实验室的研究人员提供教育和培训服务。 技术提供 TCMF为研究者提供五项具体的技术服务： 原核显微注射 ES细胞电穿孔 ES细胞注射（ESM） 线推导（DER） 胚胎冷冻保存（CRY）