CD1-restricted T cell response in atherosclerosis

动脉粥样硬化中 CD1 限制性 T 细胞反应

• 批准号: 6781860
• 负责人: Yong-Jian Geng
• 金额: $ 43.93万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6781860
• 关键词: CD1 molecule; T lymphocyte; antigen presentation; antigen receptors; atherosclerosis; autoantigens; cellular pathology; clinical research; cytokine; gene mutation; genetic polymorphism; genetically modified animals; human subject; laboratory mouse; lipids; natural killer cells; protein biosynthesis; systemic lupus erythematosus

DESCRIPTION (provided by applicant): The cluster of differentiation I (CD1) proteins represent a new class of antigen-presenting molecules, which structurally resemble MHC class I antigens and may restrict certain T lymphocyte responses to lipid antigens. There are two groups of CD1 molecules encoded by five CD1 genes (CD1a, b, c, d and e). Atherosclerotic lesions contain numerous T cells and CD1-positive macrophages and dendritic cells in addition to chemically modified lipids (e.g., cholesterol oxides or oxysterols and oxidized phospholipids) present in oxidized lipoproteins, raising the possibility that CD1-restricted T cells may exist in the lesions and respond to atherogenic lipid antigens presented by local macrophages and dendritic cells. The specific aims of this proposal are to determine (1) the CD1 isotype expression and CD1-restricted T cell response to lipid components of oxidized lipoproteins in atherosclerosis; (2) the association of an CD1 mutation or polymorphism with altered vascular autoimmunity in patients with SLE and atherosclerosis; (3) the cytokine production and pro-apoptotic function of CD1d-restricted NK T cells that are reactive to lipid antigens; and (4) the impact of knocking out CD1d gene on the T cell responses to lipid antigens and the development of atherosclerosis caused by apolipoprotein-E deficiency. The experimental procedures involve the analysis of T cell cytokine production and antigen receptor profiles, the evaluation of T cell response to CD1- lipid complexes, the assessment of genomic DNA sequences for CD1 genes, and the histopathological characterization of atherosclerotic lesions in the CD1/apolipoprotein-E double knockout mice recently established in our lab. Accomplishment of this project is anticipated to generate valuable information that help understand the immune mechanisms underlying vascular injury during atherogenesis.

描述（由申请人提供）：

项目成果

MicroRNA-221 regulates high glucose-induced endothelial dysfunction.

• DOI: 10.1016/j.bbrc.2009.02.013
• 发表时间: 2009-03-27
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Li, Yangxin;Song, Yao-Hua;Li, Fan;Yang, Tong;Lu, Yao Wei;Geng, Yong-Jian
• 通讯作者: Geng, Yong-Jian

Inhibition of Gata4 and Tbx5 by Nicotine-Mediated DNA Methylation in Myocardial Differentiation.

尼古丁介导的 DNA 甲基化对心肌分化中 Gata4 和 Tbx5 的抑制

• DOI: 10.1016/j.stemcr.2016.12.016
• 发表时间: 2017-02-14
• 期刊: Stem cell reports
• 影响因子: 5.9
• 作者: Jiang XY;Feng YL;Ye LT;Li XH;Feng J;Zhang MZ;Shelat HS;Wassler M;Li Y;Geng YJ;Yu XY
• 通讯作者: Yu XY

Characterization of a novel ubiquitin-conjugating enzyme that regulates beta1,4-galactosyltransferase-1 in embryonic stem cells.

一种调节胚胎干细胞中 β1,4-半乳糖基转移酶-1 的新型泛素结合酶的表征。

• DOI: 10.1634/stemcells.2007-1080
• 发表时间: 2008
• 期刊: Stem cells (Dayton, Ohio)
• 作者: Wassler,MichaelJ;Shur,BarryD;Zhou,Wenxia;Geng,Yong-Jian
• 通讯作者: Geng,Yong-Jian