MOLECULAR AND CELLULAR BIOLOGY

分子和细胞生物学

• 批准号: 6813360
• 负责人: BRUCE C. KONE
• 金额: $ 12.01万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-06 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6813360
• 关键词: animal tissue; biomedical facility; cell biology; gastrointestinal disorder; genetic techniques; human tissue; immunologic assay /test; microscopy; molecular biology; multiple organ failure; trauma

The discovery of novel mechanisms and therapies for multiple organ failure, as proposed in the individual subprojects of this Center, requires sophisticated cellular and molecular biological approaches, reagents, and methods. The Cellular and Molecular Biology Core represents a centralized area of research expertise, dedicated laboratory space, specialized equipment, and training for the Center designed to provide Center investigators and their collaborators cost-effective access to the tools and services they need to accomplish their specific aims. The Aim of the Core is to develop and provide to Trauma Center investigators cellular and molecular reagents, methodology, specialized equipment, and training for the detection characterization, localization, and quantitation of specific gene products and non-genomic mediators, and for assessments of cell and tissue morphology. It supports the needs of the individual subprojects for cellular and molecular reagents, imaging, experimental design, data interpretation, and specific methods relevant to their projects. Technical capabilities of the Core include quantitative RT-PCR, Northern analysis, RNase protection assays, primer extension assays, gene knockdown, Western blotting, immunoprecipitation, genotyping, transgenic mice production, antibody and molecular probe production, EMSA, DNase I footprinting, transcription assays, immunofluorescence microscopy, histology, immunohistochemistry, TUNEL assays, other recombinant DNA methods, 2D gel electrophoresis, DNA microarray analysis, in vitro and in vivo gene transfer, and superoxide, myeloperoxidase, and S-nitrosylation assays. Training academic surgery residents in modern molecular techniques of investigation remains a primary mission of the Core. Successful attainment of these goals will enhance the productivity of the investigators and make available to the scientific community new tools. The core has an integrated administrative and budgetary structure to utilize efficiently resources and maintain standardization of methods, quality control, and control of reagent/service utilization.

正如该中心的各个子项目所提出的，发现多器官衰竭的新机制和疗法需要复杂的细胞和分子生物学方法、试剂和方法。 细胞和分子生物学核心代表了中心的研究专业知识、专用实验室空间、专业设备和培训的集中领域，旨在为中心研究人员及其合作者提供实现特定目标所需的工具和服务，具有成本效益。核心的目标是开发并向创伤中心研究人员提供细胞和分子试剂、方法、 专门的设备和培训，用于特定基因产物和非基因组介质的检测表征、定位和定量，以及细胞和组织形态的评估。它支持各个子项目对细胞和分子试剂、成像、实验设计、数据解释以及与其项目相关的特定方法的需求。核心技术能力包括定量RT-PCR、Northern 分析、RNase 保护测定、引物延伸测定、基因敲除、蛋白质印迹、免疫沉淀、基因分型、转基因小鼠生产、抗体和分子探针生产、EMSA、DNase I 足迹、转录测定、免疫荧光显微镜、组织学、免疫组织化学、TUNEL 测定、其他重组 DNA 方法、2D 凝胶 电泳、DNA 微阵列分析、体外和体内基因转移以及超氧化物、髓过氧化物酶和 S-亚硝基化测定。对学术外科住院医师进行现代分子研究技术培训仍然是该核心的首要任务。成功实现这些目标将提高研究人员的生产力，并为科学界提供新的工具。该核心具有综合的行政和预算结构，以有效利用资源并保持方法、质量控制和试剂/服务使用控制的标准化。