Core--Toxic Algal Culture

核心--毒藻培养

• 批准号: 6751039
• 负责人: KATHLEEN S REIN
• 金额: $ 3.41万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6751039
• 关键词: Dinoflagellates; algae; aquaculture; biomedical facility; flow cytometry; gene expression; marine toxins; microorganism mass culture; sample collection; tissue /cell culture

The proposed Toxic Algal Culture Core Facility will be located at Florida International University (FLU). This Facilities Core will provide field and laboratory support to the Toxic HAB research project (Brand/Rein), and the HAB Functional Genomics research project (Crawford/Hitchcock) as well as future Pilot Projects and other Oceans and Human Health investigators. Support will be in the form of the following; (1) Sample collection support and shipboard analysis. Continuous-flow bow pump water collection systems will be used to collect samples for flow-through chlorophyll fluorometry and flow cytometry. (2) Isolation of total population DNA from field collected samples for the preparation of 16S and 23S libraries to assess population diversity. (3) Isolation of RNA from field collected samples for gene expression studies. (4) Establishment of uni-algal and axenic (whenever possible) cultures of phytoplankton (dinoflagellates, prymnesiophytes and raphidophyts) by both traditional manual and automated cell-sorting flow cytometry methods. (5) Maintenance of clonal cultures and large scale culturing. (6) Collection of biomass from large scale cultures. (7) Growth of microorganisms under a variety of conditions for gene expression studies.

拟议的有毒藻类培养核心设施将位于佛罗里达国际大学（FLU）。该设施核心将为有毒有害藻华研究项目（Brand/Rein）、有害藻华功能基因组学研究项目（Crawford/Hitchcock）以及未来的试点项目和其他海洋与人类健康研究人员提供现场和实验室支持。支持将以以下形式提供：