Core--Toxic Algal Culture

拟议的有毒藻类培养核心设施将设在佛罗里达国际大学(Flu)。该设施核心将为有毒的HAB研究项目(Brand/Rein)和HAB功能基因组研究项目(Crawford/Hitchcock)以及未来的试点项目和其他海洋和人类健康调查人员提供现场和实验室支持。支助将以下列形式提供； (1)样品采集支持和船上分析。将使用连续流弓形泵水收集系统来收集样品，以进行流通式叶绿素荧光法和流式细胞术。 (2)从野外采集的样品中提取总的群体DNA，以备16S和23S文库，以评估群体多样性。 (3)从野外采集的样本中提取RNA，用于基因表达研究。 (4)用传统的人工和自动细胞分选流式细胞术方法建立浮游植物(甲藻、鞭藻和藻类)的单藻和无菌(尽可能)培养。 (5)保持无性系培养和规模化培养。 (6)规模化培养生物量的收集。 (7)微生物在各种条件下的生长情况，用于基因表达研究。

The proposed Toxic Algal Culture Core Facility will be located at Florida International University (FLU). This Facilities Core will provide field and laboratory support to the Toxic HAB research project (Brand/Rein), and the HAB Functional Genomics research project (Crawford/Hitchcock) as well as future Pilot Projects and other Oceans and Human Health investigators. Support will be in the form of the following; (1) Sample collection support and shipboard analysis. Continuous-flow bow pump water collection systems will be used to collect samples for flow-through chlorophyll fluorometry and flow cytometry. (2) Isolation of total population DNA from field collected samples for the preparation of 16S and 23S libraries to assess population diversity. (3) Isolation of RNA from field collected samples for gene expression studies. (4) Establishment of uni-algal and axenic (whenever possible) cultures of phytoplankton (dinoflagellates, prymnesiophytes and raphidophyts) by both traditional manual and automated cell-sorting flow cytometry methods. (5) Maintenance of clonal cultures and large scale culturing. (6) Collection of biomass from large scale cultures. (7) Growth of microorganisms under a variety of conditions for gene expression studies.