Core--Toxic Algal Culture

拟议的有毒藻类培养核心设施将设在佛罗里达国际大学（FLU）。该设施核心将为有毒有害藻华研究项目（布兰德/雷恩）、有害藻华功能基因组学研究项目（克劳福德/希区柯克）以及未来的试点项目和其他海洋与人类健康研究人员提供现场和实验室支持。支持将采取以下形式： (1)样品收集支持和船上分析。连续流船首泵水收集系统将用于收集样品，用于流式叶绿素荧光测定法和流式细胞术。 (2)从田间采集的样品中分离总种群DNA，用于制备16 S和23 S文库，以评估种群多样性。 (3)从田间收集的样品中分离RNA用于基因表达研究。 (4)通过传统的手动和自动化细胞分选流式细胞术方法建立浮游植物（甲藻，prymnesiophytes和rhaphidophytes）的单藻和无菌（只要可能）培养物。 (5)克隆培养和大规模培养的维持。 (6)从大规模培养物中收集生物质。 (7)微生物在各种生长条件下的基因表达研究。

The proposed Toxic Algal Culture Core Facility will be located at Florida International University (FLU). This Facilities Core will provide field and laboratory support to the Toxic HAB research project (Brand/Rein), and the HAB Functional Genomics research project (Crawford/Hitchcock) as well as future Pilot Projects and other Oceans and Human Health investigators. Support will be in the form of the following; (1) Sample collection support and shipboard analysis. Continuous-flow bow pump water collection systems will be used to collect samples for flow-through chlorophyll fluorometry and flow cytometry. (2) Isolation of total population DNA from field collected samples for the preparation of 16S and 23S libraries to assess population diversity. (3) Isolation of RNA from field collected samples for gene expression studies. (4) Establishment of uni-algal and axenic (whenever possible) cultures of phytoplankton (dinoflagellates, prymnesiophytes and raphidophyts) by both traditional manual and automated cell-sorting flow cytometry methods. (5) Maintenance of clonal cultures and large scale culturing. (6) Collection of biomass from large scale cultures. (7) Growth of microorganisms under a variety of conditions for gene expression studies.