Discovery of antimicrobial natural products made by Trichoderma spp. and study of their biosynthesis

木霉属抗菌天然产物的发现。

基本信息

批准号：
2391796
负责人：
金额：
--
依托单位：
University of Warwick
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2020
资助国家：
英国
起止时间：
2020 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2391796
关键词：
Discovery antimicrobial natural products made

项目摘要

Papaya (Carica papaya L.) plantations around the world are being devastated by papaya dieback disease caused by Erwinia mallotivora infections and lead to plant death. Biocontrol measures are the most promising to explore as other strategies such as crop management, chemical treatment or the use of resistant plants are either not manageable or not available. Three strains from the genus Trichoderma have been identified as active against E. mallotivora. This project has for aim to purify and characterise secondary metabolites from those three Trichoderma strains showing bioactivity against E. mallotivora. To do so, the first step will be to purify all major metabolites made by all three strains using HPLC and testing their activity on Gram-negative bacteria, such as Escherichia coli and E. mallotivora. NMR spectroscopy experiments will then be performed on a few metabolites which show strong antimicrobial activity against E. mallotivora to characterise them and elucidate their structure. Once characterised, the genomes of the three strains will be explored to identify candidate BGCs responsible for their production. This will be facilitated as AntiSMASH detection of BGCs has already been performed. After identification of the BGCs, work with CRISPR/Cas9 deletions or heterologous expression coupled with LC/MS analysis and NMR spectroscopy experiments will be performed to elucidate the role of each gene present in the cluster.

世界各地的木瓜（Carica Papaya L.）种植园被Erwinia Mallotivora感染引起的木瓜木dieback疾病遭受了破坏，并导致植物死亡。由于作物管理，化学处理或使用抗性植物的使用是不可管理的或不可用的，因此生物防治措施是最有前途的探索。来自毛del虫属的三种菌株已被鉴定为对毛托曲霉的活性。该项目的目的是纯化和表征来自这三个毛d虫菌株的二级代谢产物，显示出针对Maltotivora的生物活性。为此，第一步将是使用HPLC净化所有三种菌株制造的所有主要代谢产物，并测试其对革兰氏阴性细菌的活性，例如大肠杆菌和大肠杆菌。然后将对一些代谢产物进行NMR光谱实验，这些代谢表现出对Malotivora的强抗菌活性，以表征它们并阐明其结构。一旦表征，将探索这三种菌株的基因组，以识别负责生产的候选BGC。这将促进，因为已经进行了对BGC的抗施剂检测。在鉴定BGC之后，将进行与CRISPR/CAS9缺失或异源表达结合结合LC/MS分析和NMR光谱实验的异源表达，以阐明群集中存在每个基因的作用。