Minority Predoctoral Fellowship Program

少数族裔博士前奖学金计划

• 批准号: 6892741
• 负责人: Juan S Ramirez-Lugo
• 金额: $ 3.99万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2006-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6892741
• 关键词: DNA damage; DNA replication; SDS polyacrylamide gel electrophoresis; Xenopus oocyte; autoradiography; cell cycle; chromosome aberrations; complementary DNA; gene expression; genetic library; genome; posttranslational modifications; predoctoral investigator; radiotracer

DESCRIPTION (provided by applicant): Genomic integrity is crucial for an organism's survival. Surveillance mechanisms ensure that the genome is properly replicated and faithfully transmitted from a cell to its daughters. If the integrity of the genome is compromised, a checkpoint mechanism is activated to delay cell cycle progression until the aberration is repaired. In particular, the DNA replication checkpoint delays progression into mitosis until the DNA has been accurately and fully replicated. While some components of the checkpoint mechanism are known, many of the elements involved in the events underlying the checkpoint response have not been characterized or are not fully understood. Using an in vitro expression cloning strategy (IVEC) to screen a Xenopus oocyte cDNA library, we wish to identify novel components and interactions involved in the DNA replication checkpoint to provide a better understanding of this vital process.

描述（由申请人提供）：基因组完整性对于生物的生存至关重要。监视机制可确保基因组得到正确复制并忠实地从细胞传播到其女儿。如果基因组的完整性受到损害，则激活检查点机制以延迟细胞周期的进程，直到修复畸变。特别是，DNA复制检查点延迟了有丝分裂的进展，直到DNA被准确而完全复制为止。尽管已知检查点机制的某些组件，但尚未表征或尚未完全了解检查点响应的事件中涉及的许多元素。使用体外表达克隆策略（IVEC）筛选爪蟾卵母细胞cDNA库，我们希望确定DNA复制检查点中涉及的新成分和相互作用，以更好地了解这一重要过程。