Protein Interactions Involved in Poxvirus Envelopment

描述（申请人提供）：无。（改编自申请书）：这项工作的主要目标是确定牛痘包膜形式特有的六种蛋白质产生包膜病毒粒子的分子机制。这六种蛋白之间的相互作用是有效包膜牛痘病毒所必需的假设将被验证。这些相互作用将通过识别包膜所需的六种蛋白质上的特定结构域来描述，并测试这些已识别的结构域与六种蛋白质的相互作用。识别这些区域以及随后它们与什么相互作用不仅可以更好地理解这六种蛋白质的功能以及它们如何协调病毒包膜，而且还将为进一步研究病毒包膜的机制奠定基础。将使用体内反式互补试验确定特定的结构域，其中被测蛋白质的特定蛋白质或结构域将在反式中提供，并使用活体荧光显微镜直观地评估互补。

DESCRIPTION (provided by applicant): None. (Adapted from the Application): The major goal of this work is to determine the molecular mechanism employed by six proteins that are unique to the enveloped form of vaccinia to produce enveloped virions. The hypothesis that interactions among these six proteins are required for efficient envelopment of vaccinia virus will be tested. These interactions will be delineated by identifying specific domains on these six proteins that are required for the envelopment and to test these identified domains for interaction with the six proteins. Identification of these regions and subsequently what they interact with will not only allow for a better understanding of the function of these six proteins and how they coordinate viral envelopment but will also lay the foundation for further studies into the mechanism of viral envelopment. Specific domains will be identified using an in vivo trans-complementation assay in which the specific protein or domains of the protein being tested will be provided in-trans and complementation will be assessed visually using live fluorescent microscopy.