Protein Interactions Involved in Poxvirus Envelopment

描述(申请人提供)：无。(改编自本申请)：这项工作的主要目标是确定牛痘病毒包膜形式特有的六种蛋白质产生包膜病毒粒子的分子机制。这六种蛋白质之间的相互作用是牛痘病毒有效包膜所必需的，这一假设将得到检验。这些相互作用将通过识别这六种蛋白质上包膜所需的特定结构域来描述，并测试这些识别的结构域与六种蛋白质的相互作用。识别这些区域及其相互作用不仅可以更好地了解这六种蛋白质的功能以及它们如何协调病毒被膜，而且还将为进一步研究病毒被膜机制奠定基础。特定的结构域将使用体内反式互补试验来鉴定，其中特定的蛋白质或被测试蛋白质的结构域将以反式方式提供，并将使用实时荧光显微镜进行目视评估。

DESCRIPTION (provided by applicant): None. (Adapted from the Application): The major goal of this work is to determine the molecular mechanism employed by six proteins that are unique to the enveloped form of vaccinia to produce enveloped virions. The hypothesis that interactions among these six proteins are required for efficient envelopment of vaccinia virus will be tested. These interactions will be delineated by identifying specific domains on these six proteins that are required for the envelopment and to test these identified domains for interaction with the six proteins. Identification of these regions and subsequently what they interact with will not only allow for a better understanding of the function of these six proteins and how they coordinate viral envelopment but will also lay the foundation for further studies into the mechanism of viral envelopment. Specific domains will be identified using an in vivo trans-complementation assay in which the specific protein or domains of the protein being tested will be provided in-trans and complementation will be assessed visually using live fluorescent microscopy.