Regulation and Function of Hox gene lin-39 in C elegans

线虫Hox基因lin-39的调控与功能

• 批准号: 6835989
• 负责人: David Eisenmann
• 金额: $ 24.99万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE COUNTY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6835989
• 关键词: Regulation; Function; Hox; gene; lin

EXCEED THE SPACE PROVIDED. The C. elegans Hox gene lin-39 encodes a homeodomain-containing protein similar to the Drosophila Deformed and Sex Combs Reduced proteins, lin-39 is expressed in the C. elegans mid-body region, including the vulval precursor cells, and is required for the proper fate determination of these cells. In these vulval precursor cells, the tin-39 gone is coordinately regulated by both Wnt and Ras signaling pathways. We propose that the Hox gene lin-39 in C. elegans functions as a transcriptional regulator that controls the expression of a set of downstream genes in order to control the fates of the vulval precursor cells. We propose experiments to 1) determine how the Wnt and Ras pathways regulate LIN-39 protein levels, 2) identify LIN-39 binding sites in vitro and assay their relevance in vivo, 3) determine whether phosphorylation alters the functions of LIN-39 in vitro or in vivo, and 4) identify and characterize target genes regulated by L1N-39 using a microarray -based approach. Hox proteins such as LIN-39 are known to function in the patterning of cells along the anterior-posterior axis during the development of all metazoans, and defects in Hox gone expression can lead to drastic defects in development. Although Hox proteins have been characterized in many species, only a handful of target genes have been identified for these proteins. Therefore much can be learned about the function of this important class of master developmental control genes in all organisms, including humans, by the characterization of Hox protein targets in the model system C. elegans, for which full genomic sequence is available and functional genomic approaches are available. PERFORMANCE SITE ========================================Section End===========================================

超出所提供的空间。秀丽隐杆线虫Hox基因lin-39编码一种含有同源结构域的蛋白，与果蝇变形蛋白和性梳蛋白相似，lin-39在秀丽隐杆线虫的身体中部表达，包括外阴前体细胞，是决定这些细胞命运的必要条件。在这些外阴前体细胞中，tin-39的消失受到Wnt和Ras信号通路的协调调节。我们认为秀丽隐杆线虫的Hox基因lin-39作为一种转录调节因子，控制一系列下游基因的表达，从而控制外阴前体细胞的命运。我们提出了以下实验：1)确定Wnt和Ras通路如何调节LIN-39蛋白水平；2)确定LIN-39的体外结合位点并分析其在体内的相关性；3)确定磷酸化是否会改变LIN-39在体外或体内的功能；4)使用基于微阵列的方法识别和表征受L1N-39调控的靶基因。已知在所有后生动物的发育过程中，诸如LIN-39之类的Hox蛋白在沿前后轴的细胞模式中起作用，Hox - go表达的缺陷会导致发育中的严重缺陷。虽然Hox蛋白在许多物种中都有特征，但只有少数的靶基因被确定为这些蛋白。因此，通过模型系统秀丽隐杆线虫中Hox蛋白靶点的表征，可以了解这类重要的主要发育控制基因在包括人类在内的所有生物中的功能，其完整的基因组序列和功能基因组方法是可用的。网站性能 ======================================== 节结束 ===========================================