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Molecular Mechanisms of Drosophila Gonad Morphogenesis

果蝇性腺形态发生的分子机制

基本信息

批准号：
6937238
负责人：
Mark B Van Doren
金额：
$ 26.34万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-07 至 2007-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6937238
关键词：
Drosophilidae arthropod genetics cadherins cell adhesion cell migration gene expression germ cells gonads histogenesis in situ hybridization membrane proteins mesoderm molecular dynamics mutant protein localization protein structure function yeast two hybrid system

项目摘要

DESCRIPTION: (provided by applicant): A fundamental problem in biology is how the germ cells develop and are nurtured by the soma so that they can give rise to the next generation of a species. This occurs largely in the gonads, where germ cells and specific somatic cells create the unique environment necessary for the germ cells to differentiate. Despite the importance of this problem. surprisingly little is known about how the germ cells and somatic cells come together to form a gonad. There are many parallels between gonad formation in Drosophila and in other species. Therefore, we can use Drosophila as a model system for understanding this process. We have discovered a new gene, fear of intimacy (foi), that is required for gonad morphogenesis in Drosophila. Infoi mutants, the cells which make up the gonad form normally but the process of gonad coalescence is blocked. FOl is predicted to be a transmembrane protein, and is a founding member of a new family of proteins conserved from yeast to humans. Interestingly, we have found that the cell adhesion molecule E-cadherin is also essential for gonad morphogenesis. Together, FOI and E-cadherin provide a unique entry point for understanding the molecular events that control the formation of the gonad. To better understand the events of gonad morphogenesis, and how FOl and E-cadherin control this process, we will carry out the following specific aims. In Aim 1 we will conduct a cell biological analysis of gonad coalescence and characterize the cellular interactions that occur during this process. In Aim 2 we will determine how FOl is acting at the cellular level to affect morphogenesis. where the FOI protein is localized and what domains of FOI are essential for its function. In Aim 3 we will analyze the role of E-cadherin in gonad morphogenesis, and determine if FOl and E-cadherin are acting together to mediate this process. The completion of these aims will provide the first comprehensive analysis of gonad morphogenesis in Drosophila and provide insight into the molecular mechanisms that control this process.

描述：（由申请人提供）：生物学的一个基本问题是如何生殖细胞发育并由索马滋养，一个物种的下一代。这主要发生在性腺中，生殖细胞和特定体细胞创造了必要的独特环境让生殖细胞分化尽管这个问题很重要。令人惊讶的是，人们对生殖细胞和体细胞是如何产生的知之甚少，一起形成生殖腺。性腺的形成与生殖腺的形成有许多相似之处，果蝇和其他物种。因此，我们可以用果蝇作为模型系统来理解这个过程。我们发现了一种新的基因，亲密度（foi），这是果蝇性腺形态发生所必需的。Infoi 突变体，组成性腺的细胞正常形成，但过程性腺的结合被阻断。F01被预测为跨膜蛋白，是一个新的蛋白质家族的创始成员，人类有趣的是，我们发现细胞粘附分子E-cadherin 也是性腺形态发生所必需的FOI和E-cadherin共同提供了一个独特的切入点，了解控制的分子事件，性腺的形成。为了更好地了解性腺形态发生的过程，以及FOl和E-钙粘蛋白如何控制这一过程，我们将进行具体目标。在目标1中，我们将进行细胞生物学分析性腺聚结和表征细胞相互作用，发生在这个过程在目标2中，我们将确定F01如何在细胞内起作用。影响形态发生。FOI蛋白定位在哪里， FOI的结构域对其功能至关重要。在目标3中，我们将分析 E-钙粘蛋白在性腺形态发生中的作用，并确定FO 1和E-钙粘蛋白共同作用于这个过程。这些目标的实现将首次对果蝇的性腺形态发生进行了全面的分析并提供对控制这一过程的分子机制的深入了解。