Dissecting actin mediated nuclear destruction during keratinocyte terminal differentiation

剖析角质形成细胞终末分化过程中肌动蛋白介导的核破坏

• 批准号: 2401701
• 金额: --
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2401701
• 关键词: Dissecting; actin; mediated; nuclear; destruction

Controlled destruction of the nucleus is required throughout the lifespan of an organism. Critical though this process is, little is known about the associated mechanisms as it is a difficult process to visualise except in epidermal keratinocytes, which undergo nuclear degradation as part of their terminal differentiation process. Using a combination of state-of the-art live high-resolution light microscopy, phosphoprotein mass spectrometry and next generation RNA sequencing approaches, the PhD student will examine the role of the actin remodelling proteins in nuclear destruction and the possibility of inducing nuclear destruction with drugs to active this actin remodelling pathway.

在生物体的整个生命周期中，需要对细胞核进行受控破坏。尽管这一过程很关键，但人们对相关机制知之甚少，因为除了表皮角质形成细胞外，这是一个很难观察的过程，作为其终末分化过程的一部分，表皮角质形成细胞经历了核降解。使用最先进的实时高分辨率光学显微镜、磷蛋白质谱仪和下一代RNA测序方法的组合，这位博士生将研究肌动蛋白重塑蛋白在核破坏中的作用，以及通过药物诱导核破坏以激活肌动蛋白重塑途径的可能性。

项目成果

Perspective: Controlling Epidermal Terminal Differentiation with Transcriptional Bursting and RNA Bodies.

• DOI: 10.3390/jdb8040029
• 发表时间: 2020-12-04
• 期刊: Journal of developmental biology
• 影响因子: 2.7
• 作者: Wotherspoon D;Rogerson C;O'Shaughnessy RFL
• 通讯作者: O'Shaughnessy RFL

Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation.

• DOI: 10.1038/s41418-020-00712-9
• 发表时间: 2021-06
• 期刊: Cell death and differentiation
• 影响因子: 12.4
• 作者: Rogerson C;Wotherspoon DJ;Tommasi C;Button RW;O'Shaughnessy RFL
• 通讯作者: O'Shaughnessy RFL