2-Photon Imaging of synaptic remodeling in visual cortex

视觉皮层突触重塑的 2 光子成像

• 批准号: 6884286
• 负责人: SUNIL P GANDHI
• 金额: $ 4.3万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6884286
• 关键词: axon; bioimaging /biomedical imaging; biological models; brain imaging /visualization /scanning; developmental neurobiology; diazepam; electromagnetic radiation; gamma aminobutyrate; genetic models; genetically modified animals; laboratory mouse; microscopy; neural inhibition; neural plasticity; neuroanatomy; postdoctoral investigator; protein isoforms; synaptogenesis; time resolved data; visual cortex; visual deprivation

DESCRIPTION (provided by applicant): This proposal aims to investigate the relationship between the anatomical and functional plasticity of mammalian brain using ocular dominance plasticity in mouse visual cortex as a model system. Occluding an eye during early postnatal development leads to a rapid loss of vision through the deprived eye. The location and means of synaptic change in ocular dominance plasticity is a major outstanding problem in systems neuroscience. Recent evidence suggests that the earliest changes occur in the superficial layers (layer ll/lll) of primary visual cortex where some connections appear to rewire on a rapid timescale (< 2 days). By using new imaging techniques that facilitate the chronic imaging of single axons in the living brain, I aim to investigate further the hypothesis that visual deprivation stimulates a rapid rearrangement of synaptic connections in layer ll/lll. At the same time, I will use intrinsic signal imaging to keep track of the ocular dominance of the cortical territory containing the axons of study. I plan to apply these techniques to study the role of inhibition in opening the critical period for ocular dominance plasticity. Altogether, it is likely that this work will help elucidate the anatomical underpinnings of cortical plasticity.

描述（由申请人提供）：本课题旨在以小鼠视觉皮层的眼优势可塑性为模型系统，探讨哺乳动物大脑的解剖可塑性与功能可塑性之间的关系。在出生后发育早期，眼睛被遮挡会导致视力迅速丧失。眼优势可塑性中突触变化的位置和方式是系统神经科学研究的一个突出问题。最近的证据表明，最早的变化发生在初级视觉皮层的浅层（第二层/第二层），在那里一些连接似乎在快速的时间尺度上（< 2天）重新连接。通过使用新的成像技术，促进活脑中单个轴突的慢性成像，我的目标是进一步研究视觉剥夺刺激第1 / 3层突触连接快速重排的假设。与此同时，我将使用固有信号成像来跟踪研究轴突所在的皮层区域的眼部优势。我计划运用这些技术来研究抑制在打开眼优势可塑性关键期中的作用。总之，这项工作很可能有助于阐明皮层可塑性的解剖学基础。