Residue-specific multidimensional IR of cyt-c folding
cyt-c 折叠的残基特异性多维红外光谱
基本信息
- 批准号:6793497
- 负责人:
- 金额:$ 4.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-07-01 至 2006-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant):
Three-pulse two-dimensional infrared spectroscopy (2D-IR) is a technique which is capable of resolving molecular structure (via angles between transition dipoles and vibrational coupling constants), dynamics (via variable time delays and homogeneous line shapes), and structural distributions on time scales down to femtoseconds. Cytochrome-c is a well-studied, fast-folding heme protein: in cyt-c folding studies, misfolded and ligand-exchanged structures have been implicated in a heterogeneous dynamic structural distribution associated with the folding events. It is proposed here to use 2D-IR to resolve the equilibrium and dynamic (folding) structural distributions of the peptide chain of cyt-c near the heme. Isotopic labeling of Met80 (a native heme ligand) and Phe82 residues with 13C/180 and 13C/160 carbonyls will be used to spectrally isolate the amide-I vibrations of this part of the chain which is close to the heine. 2D-IR of the labeled residues, which yields the distances and angles between them, will be used to determine the equilibrium structural distribution of native-sequence cyt-c and the perturbed equilibrium distributions of Phe82 mutants. After integration of a visible trigger pulse into the existing three-pulse infrared optical bench, denatured CO-heme cyt-c will be photolyzed to initiate folding to the native structure. 2D-IR of the labeled residues will be used to monitor the time-evolving structural distribution associated with cyt-c folding.
描述(由申请人提供):
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CASEY H LONDERGAN其他文献
CASEY H LONDERGAN的其他文献
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{{ truncateString('CASEY H LONDERGAN', 18)}}的其他基金
Cyanylated cysteine as vibrational probe of binding-induced structural changes in
氰化半胱氨酸作为结合诱导结构变化的振动探针
- 批准号:
8232863 - 财政年份:2009
- 资助金额:
$ 4.3万 - 项目类别:
Residue-specific multidimensional IR of cyt-c folding
cyt-c 折叠的残基特异性多维红外光谱
- 批准号:
6899693 - 财政年份:2004
- 资助金额:
$ 4.3万 - 项目类别:
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