Investigating of a Novel Checkpoint in S. Cerevisiae

描述(由申请人提供)： 以前的工作表明，起源缺陷的YAC(5ORIdeltaYAC)诱导了一个新的检查点。这个检查站不受DNA损伤的影响。它也不同于HU诱导的复制检查点，因为复制分叉不会停止，Rad53也不会被激活。因此，人们推测这个新的检查点监控着正在进行的DNA合成。有趣的是，RAD9被发现对维持这一检查点是必不可少的，这表明在正常细胞周期中，RAD9在监控S期进展中所起的作用是未知的。将进行基因筛查，以确定这一新的检查点途径的其他组成部分。这项工作将有助于我们理解检查点机制在不受干扰的细胞周期中是如何运作的。这也将进一步加深我们对癌细胞如何逃脱细胞周期和检查点控制，导致基因组不稳定的理解。

DESCRIPTION (provided by applicant): Previous work has shown that an origin-deficient YAC (5ORIdeltaYAC) induces a novel checkpoint. This checkpoint is independent of DNA damage. It is also different from the HU-induced replication checkpoint because replication forks do not stall and Rad53 is not activated. Therefore, it was postulated that this novel checkpoint monitors ongoing DNA synthesis. Interestingly, Rad9 was found essential for maintaining this checkpoint, indicating a previously unknown role of Rad9 in monitoring S phase progression in a normal cell cycle. A genetic screen will be conducted to identify additional components of this novel checkpoint pathway. This work will contribute to our understanding of how checkpoint mechanisms operate during an unperturbed cell cycle. It will also further our understanding of how cancer cells escape cell cycle and checkpoint control, leading to genome instability.