Effects of Physical Activity on Hippocampal Neurogenesis

描述（由申请人提供）：在整个成年期，大脑在海马体中继续产生新的神经元。神经发生过程受学习、压力、空间复杂性和身体活动等环境因素的影响。本研究将探讨轮跑活动对神经发生的控制，并研究这一现象的时间和生理参数。此外，将使用强制跑步机和自愿跑步轮活动来检查锻炼的意志方面。胸腺嘧啶类似物5-溴脱氧尿嘧啶（BrdU）通常用于海马细胞增殖和存活的研究。然而，在研究提升循环系统的环境因素时，可用性问题导致结果难以解释。为此，内源性标志物Ki-67和磷酸化组蛋白H3的免疫标记将用于提供不受全身升高影响的运动诱导神经发生的测量。在老年人中，体育活动与较低的痴呆症发病率相关，并且来自运动诱导的神经发生研究的数据可能有一天被应用于预防或治疗神经退行性疾病。

DESCRIPTION (provided by applicant): The brain continues to generate new neurons in the hippocampus throughout adulthood. The process of neurogenesis is influenced by environmental factors such as learning, stress, spatial complexity, and physical activity. The proposed research will explore the control of neurogenesis by wheel running activity, and investigate the temporal and physiological parameters of this phenomenon. In addition, the volitional aspect of exercise will be examined using forced treadmill and voluntary running wheel activity. The thymidine analog 5-bromodeoxyuridine (BrdU) is typically used in studies of cell proliferation and survival in the hippocampus. However, when studying environmental factors that elevate the circulatory system, the issue of availability causes difficulty in interpretation of results. To this end, immunolabeling for the endogenous markers Ki-67 and phospho-histone H3 will be applied to provide a measure of exercise-induced neurogenesis that is not affected by systemic elevation. Physical activity has been correlated with a lower incidence of dementia in older adults, and the data from studies of exercise-induced neurogenesis may one day be applied to the prevention or treatment of neurodegenerative disease.