Growth factor gene mediated cartilage repair

生长因子基因介导的软骨修复

• 批准号: 6656384
• 负责人: Laurie Goodrich
• 金额: $ 5.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-14 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6656384
• 关键词: articular cartilage; cartilage development; cartilage metabolism; cell transplantation; gene therapy; horses; insulinlike growth factor; musculoskeletal regeneration; nonhuman therapy evaluation; postdoctoral investigator; transfection /expression vector

DESCRIPTION (provided by applicant): The aim of the proposed research is to develop and validate a gene therapy protocol for the sustained local release of Insulin-Like Growth Factor-I (IGF-I) in amounts sufficient to promote healing of equine articular cartilage. IGF-I has an important role in cartilage development and homeostasis, and in previous studies has been shown to promote healing in experimentally injured cartilage. An important limitation, however, has been the inability to sustain therapeutic levels of IGF-I at the site of injury. To overcome this limitation, I would use an adenovirus vector to introduce an IGF-I gene construct into equine chondrocytes. The transfected cells would then be imported into experimentally injured joints, with the resulting repair tissue examined at predetermined intervals. Thereafter, I would assess donor cell survival and quantify local IGF-I gene expression, correlations being drawn with biomechanical, molecular biological, histological and biomechanical analyses that reflect various aspects of the healing process.

描述（由申请人提供）：拟议研究的目的是开发和验证一种基因治疗方案，用于持续局部释放胰岛素样生长因子-I（IGF-I），其量足以促进马关节软骨愈合。IGF-I在软骨发育和体内平衡中具有重要作用，并且在先前的研究中已显示出促进实验性损伤软骨的愈合。 然而，一个重要的限制是不能在损伤部位维持治疗水平的IGF-I。为了克服这一局限性，我将使用腺病毒载体将IGF-I基因构建体导入马软骨细胞。然后将转染的细胞导入实验性损伤的关节，并在预定的时间间隔内检查由此产生的修复组织。此后，我将评估供体细胞存活率并量化局部IGF-I基因表达，与反映愈合过程各个方面的生物力学，分子生物学，组织学和生物力学分析相关。

项目成果

Extracellular vesicles in the treatment and prevention of osteoarthritis: can horses help us translate this therapy to humans?

• DOI: 10.20517/evcna.2023.11
• 发表时间: 2023-06
• 期刊: Extracellular vesicles and circulating nucleic acids
• 作者: O'Brien, Thomas J.;Hollinshead, Fiona;Goodrich, Laurie R.
• 通讯作者: Goodrich, Laurie R.