Identification of type III secreted Chlamydia proteins

III 型分泌型衣原体蛋白的鉴定

• 批准号: 6914521
• 负责人: THERESA D HO
• 金额: $ 2.81万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6914521
• 关键词: Chlamydia trachomatis; Escherichia coli; MHC class I antigen; Salmonella typhimurium; adenylate cyclase; antigen presentation; bacteria infection mechanism; bacterial proteins; bactericidal immunity; binding sites; cell line; chlamydial disease; cyclic AMP; cytotoxic T lymphocyte; flow cytometry; fusion gene; gene expression; genetic library; polymerase chain reaction; protein localization; protein signal sequence; protein structure function; protein transport; recombinant proteins; reporter genes; secretory protein; vaccinia virus

DESCRIPTION (provided by applicant): A growing body of evidence suggests that Chlamydiae encode a type III secretion system to translocate bacterial proteins into the host cell. Many studies of type III secreted proteins from other organisms use a truncated adenylate cyclase protein (CyaA) with no endogenous translocation domain to demonstrate that a protein under study is a substrate for a type III secretion system. This study will use a similar approach to identify Chiamydia trachomatis proteins that are translocated into the host cell cytoplasm via a type III secretion system. These type III secreted C. trachomatis proteins will be further characterized by determining where the proteins localize during C. trachomatis infection and how these proteins are regulated. Because the type III secreted Chiamydia proteins may be translocated into the host cell cytoplasm, the study will also demonstrate 1) if the C. trachomatis proteins are processed and presented by MHC-I in C. trachomatis-infected cells in vitro and 2) whether the CD8+ T cell responses to secreted C. trachomatis proteins can provide protection against a C. trachomatis challenge in vivo. Determination and characterization of translocated C. trachomatis proteins may help to identify targets for immune interventions or theraputic treatment of C. trachomatis infection.

描述（由申请人提供）：越来越多的证据表明 衣原体编码一个III型分泌系统以易位细菌蛋白 进入宿主单元。许多来自其他的III型分泌蛋白的研究 生物体使用无内源性的截短的腺苷酸环化酶蛋白（CYAA） 转运域证明正在研究的蛋白质是底物 对于III型分泌系统。这项研究将使用类似的方法 识别易解于宿主的chiamydia沙眼蛋白 通过III型分泌系统的细胞细胞质。这些类型III分泌C. 沙眼瘤蛋白将通过确定在哪里来进一步表征 蛋白质在沙眼梭状芽孢杆菌感染期间定位，这些蛋白质是如何定位的 受监管。因为III型分泌的chiamydia蛋白可能会被转移 进入宿主细胞细胞质，该研究还将证明1）如果C. MHC-I在C中处理和提出了沙眼蛋白。 体外感染的气管体感染细胞和2）CD8+ T细胞是否反应 分泌的梭状芽胞庭蛋白质蛋白可以提供针对C的保护。 气管体挑战体内。决心和表征 易位的梭状疱疹蛋白质蛋白可能有助于识别免疫的靶标 干预措施或对沙眼衣原体感染的治疗治疗。