Development of new DNA-binding sensitisers for biosensors

开发用于生物传感器的新型 DNA 结合敏化剂

• 批准号: 2417325
• 金额: --
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2417325
• 关键词: Development; new; DNA; binding; sensitisers

The aim of this project is to develop new molecules for use as DNA-binding sensitisers in biosensors. The project will develop the synthesis of functional novel organic and inorganic molecules and use biophysical methods to fully describe their DNA-binding behaviour.The project will involve organic and inorganic synthesis, and characterisation using a breadth of analytical and spectroscopic techniques including NMR, UV-vis., fluorescence, circular dichroism, and electron paramagnetic resonance as well as titration calorimetry. There is extensive scope for developing new materials and evolving their applications.The project will involve:the design, synthesis and characterisation of new so-called sensitisers, i.e. DNA-binding molecules with spectroscopic properties allowing sensitive detection biophysical studies of the interactions of the new molecules with nucleic acid structures, including DNA*DNA duplexes.evaluation of the detection of pathogen DNA by a prototype biosensor.The envisaged applications are in sensitive and robust point-of-care biosensors for rapid detection of pathogens by their DNA using new detection approaches.

该项目的目的是开发新的分子，用作生物传感器中的DNA结合敏化剂。该项目将开发功能性新型有机和无机分子的合成，并使用生物物理方法充分描述其DNA结合行为。该项目将涉及有机和无机合成，以及使用广泛的分析和光谱技术（包括NMR，UV-vis）进行表征。荧光、圆二色性和电子顺磁共振以及滴定量热法。开发新材料和发展其应用的范围很广。该项目将涉及：设计、合成和表征新的所谓敏化剂，即具有光谱特性的DNA结合分子，允许灵敏地检测新分子与核酸结构（包括DNA*DNA双链体）的相互作用的生物物理研究。评估原型生物传感器对病原体DNA的检测。设想的应用是灵敏和稳健的即时生物传感器，用于使用新的检测方法通过DNA快速检测病原体。