Development of new DNA-binding sensitisers for biosensors

该项目的目的是开发用于生物传感器中dna结合敏化剂的新分子。该项目将开发功能新颖的有机和无机分子的合成，并使用生物物理方法来全面描述它们的dna结合行为。该项目将涉及有机和无机合成，并使用广泛的分析和光谱技术进行表征，包括NMR， UV-vis。荧光、圆二色性、电子顺磁共振以及滴定量热法。开发新材料和发展其应用具有广泛的空间。该项目将涉及：设计、合成和表征新的所谓的致敏剂，即具有光谱特性的DNA结合分子，可以对新分子与核酸结构（包括DNA*DNA双链）的相互作用进行灵敏的检测和生物物理研究。生物传感器原型检测病原体DNA的评价。设想的应用是在敏感和强大的即时护理生物传感器中，使用新的检测方法通过DNA快速检测病原体。

The aim of this project is to develop new molecules for use as DNA-binding sensitisers in biosensors. The project will develop the synthesis of functional novel organic and inorganic molecules and use biophysical methods to fully describe their DNA-binding behaviour.The project will involve organic and inorganic synthesis, and characterisation using a breadth of analytical and spectroscopic techniques including NMR, UV-vis., fluorescence, circular dichroism, and electron paramagnetic resonance as well as titration calorimetry. There is extensive scope for developing new materials and evolving their applications.The project will involve:the design, synthesis and characterisation of new so-called sensitisers, i.e. DNA-binding molecules with spectroscopic properties allowing sensitive detection biophysical studies of the interactions of the new molecules with nucleic acid structures, including DNA*DNA duplexes.evaluation of the detection of pathogen DNA by a prototype biosensor.The envisaged applications are in sensitive and robust point-of-care biosensors for rapid detection of pathogens by their DNA using new detection approaches.