Development of new DNA-binding sensitisers for biosensors

该项目的目的是开发新的分子，用作生物传感器中的DNA结合敏感剂。该项目将开发合成新型有机和无机功能分子，并使用生物物理方法来全面描述它们与DNA的结合行为。该项目将涉及有机和无机合成，以及使用广泛的分析和光谱技术进行表征，包括核磁共振、UV-Vis、荧光、圆二色谱、电子顺磁共振和滴定量热法。该项目将涉及：设计、合成和表征新的所谓敏感剂，即具有光谱特性的DNA结合分子，可以灵敏地检测新分子与核酸结构(包括DNA*DNA双链)的相互作用。评估原型生物传感器对病原体DNA的检测。设想的应用是在灵敏和坚固的护理点生物传感器中，利用新的检测方法通过DNA快速检测病原体。

The aim of this project is to develop new molecules for use as DNA-binding sensitisers in biosensors. The project will develop the synthesis of functional novel organic and inorganic molecules and use biophysical methods to fully describe their DNA-binding behaviour.The project will involve organic and inorganic synthesis, and characterisation using a breadth of analytical and spectroscopic techniques including NMR, UV-vis., fluorescence, circular dichroism, and electron paramagnetic resonance as well as titration calorimetry. There is extensive scope for developing new materials and evolving their applications.The project will involve:the design, synthesis and characterisation of new so-called sensitisers, i.e. DNA-binding molecules with spectroscopic properties allowing sensitive detection biophysical studies of the interactions of the new molecules with nucleic acid structures, including DNA*DNA duplexes.evaluation of the detection of pathogen DNA by a prototype biosensor.The envisaged applications are in sensitive and robust point-of-care biosensors for rapid detection of pathogens by their DNA using new detection approaches.