UNIVERSITY OF ARIZONA FOR INITIATIVE FOR MINORITY PHD'S

这是一个哈佛-布兰代斯联合项目，目标包括方法学发展和细胞生物学应用。四个主要目标是：(1)通过对接和精炼电子冷冻显微镜(CryoEM)分子图中组成部分的原子模型来获得大结构原子模型的先进方法；(2)提高我们可以从单个粒子的CryoEM获得的分子图的精度和分辨率，通过开发分子替换和相延伸方法来加速2D晶体结构的确定；(4)改进来自CryoEM的分子图的使用，以启动大型组装研究中的相确定。通过X射线结晶学。有四个主要项目，三个核心，以及一套相关项目。主要和相关的项目侧重于下列生物系统：(1)肌动蛋白细胞骨架；(2)病毒和病毒进入细胞；(3)膜蛋白，特别是MIP和MAPEG家族的成员；(4)抗菌肽合成酶；(5)剪接体组件；(6)T7期复制复合体(“复制体”)。这些核心是合作努力的核心组成部分，支持共享最先进的电子显微镜设施、软件开发和财团的管理。

This is a joint Harvard-Brandeis program project, with goals that include both methodological developments and cell-biological applications. The four major goals are: (1) advance methods for obtaining atomic models of large structure by docking and refining atomic models of the component parts in molecular maps from electron cryomicroscopy (cryoEM); (2) improve the accuracy and resolution of the molecular maps we can obtain from cryoEM of single particles, accelerate 2D crystal structure determination, through development of molecular replacement and phase extension methods; (4) improve use of molecular maps from cryoEM to initiate phase determination in studies of large assemblies. By x-ray crystallography. There are four major projects, three cores, and a set of associated projects. The major and associated projects focus on the following biological systems: (1) the actin cytoskeleton; (2) viruses and viral entry into cells; (3) membrane proteins, especially members of the MIP and MAPEG families; (4) antibiotic peptide synthetases; (5) spliceosome assemblies; (6) the phase T7 replication complex, ("replisome"). The cores, which are a central component of the collaborative effort, support shared state-of-the-art electron microscope facilities, software development, and administration of the consortium.