Core-- Biomarkers

核心--生物标志物

• 批准号: 6991770
• 负责人: Janine G Einspahr
• 金额: $ 35.66万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6991770
• 关键词: animal tissue; biomarker; biomedical facility; clinical research; histopathology; human tissue; immunocytochemistry; method development; sample collection; skin neoplasms; tissue /cell preparation; tissue resource /registry

DESCRIPTION (provided by applicant): The Biomarkers Core will serve as a collection and distribution point for blood and tissue samples related to Projects I, II, III, and IV as well as Core D. It will provide a resource and service for carrying out the laboratory analyses proposed in this application in support of the basic science and clinical trials. The Core will develop and be responsible for the appropriate sample collection, logging, tracking, storage, sample preparation procedures, sample processing, and distribution for all animal and human samples collected during the course of this proposal. It also will be responsible for the training of personnel involved in the collection and processing of study samples. In addition, the Core will be responsible for developing immunohistochemical and analytical methods for the measurement of targets from Projects I, II, III, and IV. The Biomarkers Core will perform assays and will work closely with the Biometry Core to provide data for reporting and interpretation. Quality assurance and quality control procedures will continue to be developed by the Biomarkers Core for sample acquisition, processing, and analysis procedures included in the proposal. The Biomarkers Core will develop and optimize methods for measuring the secondary laboratory-directed research endpoints proposed in each project. Endpoints to be analyzed by the Biomarkers Core include blood and skin levels of chemoprevention agents and micronutrients, histopathology and karyometric measurements on skin biopsies as well as immunohistochemical assays for cell proliferation, apoptosis, p53 protein expression, retinoid receptors, and other targets identified within the proposal.

描述（由申请人提供）：生物标志物核心将用作收藏 与项目I，II，III和IV以及CoreD有关的血液和组织样本的分布点以及CoreD。它将提供资源和服务，以进行本应用中提出的实验室分析，以支持基础科学和临床试验。核心将开发并负责适当的样品收集，记录，跟踪，存储，样本准备程序，样品处理以及在本提案过程中收集的所有动物和人类样品的分布。它也将负责培训参与研究样本的收集和处理的人员。此外，核心将负责开发免疫组织化学和分析方法，以测量项目I，II，III和IV项目。生物标志物核心将执行测定法，并将与生物特征核心紧密合作，以提供用于报告和解释的数据。质量保证和质量控制程序将由提案中包括的样本获取，处理和分析程序的生物标志物核心继续开发。生物标志物核心将开发并优化用于测量每个项目中提出的二级实验室指导研究终点的方法。生物标志物核心要分析的终点包括化学预防剂的血液和皮肤水平，以及微量营养素的血液和皮肤病理学，组织病理学和核测量测量以及对细胞增殖，凋亡，凋亡，p53蛋白质表达，视网膜素的受体以及其他靶标的细胞增殖，凋亡，凋亡的免疫组织化学测定。