CORE--Microarray and Bioinformatics

该核心B的微阵列部分将为鼠疫耶尔森菌、土拉菌弗朗西斯菌和大肠杆菌的基因组制造斑点DNA微阵列。此外，该设施将根据需要定制多达5000个基因的人体微阵列。该设施还将提供设备，用荧光扫描仪和计算机对阵列进行初步分析，并将数据存入生物信息学设施维护的数据库。微阵列设备将使用Affymetrix基因芯片和已经存在于石溪的Affymetrix基因芯片扫描仪。Affymetrix芯片将用于分析哺乳动物细胞对本项目中所代表的三种A类病原体感染的反应。分析

The Microarray portion of this Core B will manufacture spotted DNA microarrays for the genomes of Yersinia pestis, Francisella tularensis, and E. coli. In addition, the facility will make custom human microarrays of up to 5,000 genes as needed. The facility will also provide equipment for the initial analysis of the arrays with fluorescent scanners and computers and will deposit the data in a database maintained by the Bioinformatics facility. The Microarray facility will have access to Affymetrix gene chips and an Affymetrix gene chip scanner already present at Stony Brook. Affymetrix chips will be used to analyze the response of mammalian cells to infection with the three Category A agents that are represented in this Project. Analysis of Affymetrix data will be essentially the same as for spotted DNA microarrays. The microarray and bioinformatics facilities of this Core will work closely together on microarray experiments. The Bioinformatics portion of the Core will maintain a cluster of computers for the use of this Project. Software suites for analysis of various kinds of genomic data will be maintained for sequence comparisons, and microarray analysis. The Facility will maintain databases for storage of microarray results, and for gene annotation of the organisms of interest. The Core will be able to provide advice and assist with the design of both spotted DNA and Affymetrix microarray experiments; it will assist in the interpretation of microarray experiments; and it will provide training, programming support, and guidance in the use of various software packages.