Core--Vector

核心--向量

• 批准号: 6775151
• 负责人: Lili Wang
• 金额: $ 31.7万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6775151
• 关键词: Adenoviridae; Lentivirus; Retroviridae; affinity chromatography; biomedical facility; enzyme linked immunosorbent assay; transfection /expression vector

The Vector Core has undergone substantial evolution during the current cycle of the Center to leverage the tremendous progress that has been made in novel viral vectors. Dr. Lili Wang was recruited from Dr. Inder Verma's laboratory to direct the Vector Core. During the last four years, the list of services provided from the Vector Core has expanded exponentially. At the time of the last renewal of the grant the core provided services with vectors based on adenovirus serotype 5, adeno-associated virus (AAV) serotype 2 and murine based ecotropic and amphotropic retroviruses. The list of services provided by the core has expanded to include: Adenovirus - vectors based on human Ad5 and at least 5 simian adenoviruses; AAV- vectors based on the human AAV serotypes 1,2 and 5 and simian AAV serotype 7 and 8 with this list expanding to include 10 additional AAV serotypes over the next cycle of the grant; lentiviruses - at least 6 different pseudotypes based on HIV and FIV; and murine retroviruses - various pseudotypes.

在本中心的当前周期中，载体核心经历了实质性的演变，以利用在新型病毒载体方面取得的巨大进展。Lili Wang博士是从Inder Verma博士的实验室招募来指导Vector Core的。在过去四年中，Vector Core提供的服务列表呈指数级增长。在最近一次更新赠款时，核心提供了基于腺病毒血清型5、腺相关病毒（AAV）血清型2和鼠嗜亲性和嗜亲性逆转录病毒的载体服务。核心提供的服务清单已扩展到包括：腺病毒-基于人Ad 5和至少5种猿猴腺病毒的载体; AAV-基于人AAV血清型1、2和5以及猿猴AAV血清型7和8的载体，该清单扩展到 包括在下一轮拨款中增加10种AAV血清型;慢病毒-至少6种基于HIV和FIV的不同假型;以及鼠逆转录病毒-各种假型。