CORE--TISSUE CULTURE/HYBRIDOMA

核心--组织培养/杂交瘤

• 批准号: 6717762
• 负责人: MARK E IRELAND
• 金额: $ 8.73万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6717762
• 关键词: biomedical facility; enzyme linked immunosorbent assay; eye; hybridomas; in situ hybridization; organ culture; tissue /cell culture; tissue /cell preparation; vision

The Tissue Culture/Hybridoma/Molecular Module, located in the Department of Anatomy/Cell Biology will provide a centralized facility for tissue and organ culture and an experienced research assistant. The research assistant will be available to help investigators who have little expertise in tissue culture to develop experimental methods relevant to their studies. In addition, she will assist investigators with the production of mAbs and in carrying out routine molecular techniques. Projects proposed requiring Module use will be presented to the Module co-director (Dr. Kurpakus-Wheater) for initial consultation. Once approved, the projects will be undertaken by either the PI proposing the work or personnel from his/her laboratory under direct supervision and with the help of the Module research assistant. The newly dedicated space and equipment for molecular studies has greatly expanded and enhanced the functions of this module. Those investigators who lack experience in the technology required for molecular biology, tissue/organ culture, or mAb production will rely heavily on the equipment and availability of the research assistant for technical advice to their projects. Those investigators knowledgeable in the technical aspects involved will use the facility to enhance their own studies and to begin pilot research projects. This centralized Core facility will thus continue to provide a reasonable, cost efficient mechanism to facilitate and enhance numerous NEI funded research projects.

位于解剖学/细胞生物学系的组织培养/杂交瘤/分子模块将提供组织和器官培养的中央设施和一名经验丰富的研究助理。研究助理将帮助在组织培养方面缺乏专业知识的研究人员开发与他们的研究相关的实验方法。此外，她还将协助研究人员生产单抗和进行常规的分子技术。需要使用模块的拟议项目将提交给模块联席主任(库尔帕库斯-怀特博士)进行初步协商。一旦获得批准，这些项目将由提出工作建议的PI或其实验室的人员在模块研究助理的直接监督下进行。新的专用空间和设备 为分子研究极大地扩展和增强了这个模块的功能。那些在分子生物学、组织/器官培养或单抗生产所需技术方面缺乏经验的研究人员将严重依赖研究助理的设备和可用性，为他们的项目提供技术建议。那些了解所涉技术方面的调查人员将利用该设施加强他们自己的研究并开始试点研究项目。因此，这个集中的核心设施将继续提供一个合理的、具有成本效益的机制，以促进和加强由NEI资助的众多研究项目。