CORE--TRANSGENIC AND KNOCKOUT MOUSE CORE

转基因小鼠的使用呈指数级增长。转基因和基因缺陷小鼠在研究糖尿病、内分泌紊乱、肥胖、糖尿病的微血管和大血管并发症以及阐明单个基因在正常生理、发育和内分泌功能中的作用等方面得到了广泛的应用，具有极其重要的价值。事实上，对老鼠的基因操作几乎应用于生物医学研究的每一个领域。它可以说是在生理学和病理生理学中剖析基因功能的最有力的工具。然而，由于在个别实验室内建立这些程序所需的高昂成本和技术复杂性，将这些技术作为核心设施予以支持是至关重要的。这需要一种高效且具有成本效益的共同设施，使个体研究人员能够同时拥有表达感兴趣基因的转基因小鼠和携带基因中断或替换的小鼠。这个核心提供的技术将这些强大的分析工具掌握在所有人的手中 我们的调查人员。当与其他由 该中心的小鼠表型鉴定、转录基因组学以及生物化学和分子分析核心设施，为我们的研究人员提供了从多个角度同时检查这些过程的无限机会。 我们目前的设施在为多个UCSD实验室创造转基因小鼠和基因敲除小鼠品系方面拥有既定的记录。向整个DERC成员提供这一核心设施将极大地促进整个南加州糖尿病/内分泌学社区的研究。如果没有这样一个集中的资源，大多数实验室就无法利用这些强大的模型，这严重限制了研究，并为我们的糖尿病社区提供了机会。UCSD的转基因和敲除小鼠核心在社区中受到广泛尊重，并被视为现代研究的重要资源。以大幅折扣率提供服务将大大提高研究的质量、数量和创造性，同时还将提高研究的成本效益和效率。

The use of genetically engineered mice has grown exponentially. Transgenic and genetically deficient mice are now widely used and extremely valuable for the study of the mechanisms of diabetes, endocrine disorders, obesity, and microvascular and macrovascular complications of diabetes as well as elucidating the roles of individual genes in normal physiology, development, and endocrine function. In fact, the genetic manipulation of mice is used in virtually every field of biomedical investigation. It is arguably the most potent tool available to dissect gene function in physiology and pathophysiology. However, because of the prohibitive costs and the technical sophistication required to establish these procedures within individual laboratories, it is essential to support these technologies as a core facility. This requires an efficient and cost-effective common facility that enables individual investigators to have both transgenic mice expressing genes of interest and mice carrying gene disruptions or substitutions created for them. The techniques available from this Core place these potent tools for analysis in the hands of all of our investigators. When used in combination with the other modern techniques supplied by the Center's Mouse Phenotyping, Transcriptional Genomics, and Biochemistry and Molecular Assay Core Core facilities, it gives our investigators unlimited opportunities to examine these processes from several perspectives simultaneously. Our current facility has an established track record of enabling the creation of both transgenic mice and knockout mouse strains for multiple UCSD laboratories. The availability of this Core facility to the entire DERC membership will greatly facilitate the research of the entire diabetes/endocrinology community in Southern California. Without such a centralized resource, most laboratories would not be in a position to utilize these powerful models, severely limiting the research and granting opportunities for our diabetes community. The Transgenic and Knock-out Mouse Core of UCSD is widely respected in the community and regarded as an essential resource for modern research. The availability of its services at substantially discounted rates will substantially improves the quality, quantity and creativity of the research, while also enhancina cost-effectiveness and efficiency.