CORE--MOUSE PHENOTYPING

小鼠表型核心将提供糖尿病啮齿动物模型表征方面的专业知识和培训。为了实现这一使命，CORE将在糖尿病小鼠模型的组织病理学特征以及基本代谢分析(如葡萄糖和胰岛素耐量测试)的执行方面提供协助。核心还将就产生重组和近交系转基因和基因敲除小鼠的适当方法提供咨询，并与基因组核心进行互动，以帮助研究人员为基因组扫描设计适当的基因分型策略。此外，核心正在进行中 发展专门技术进行葡萄糖钳夹研究，以调查全身和器官特定的代谢参数。通过与拟议的DERC的其他核心合作，可以从啮齿动物对新陈代谢、摄食行为、能量平衡、身体成分和内分泌功能的影响方面，彻底表征已定义的基因改变的影响。核心将为许多DERC研究人员提供关于如何防止育种计划中常见错误的建议，从而为他们提供重要的培训和教育功能。

The mouse phenotyping core will provide expertise and training in the characterization of rodent models of diabetes. To achieve this mission, the Core will provide assistance in the histopathological characterization of mouse models of diabetes and training in the performance of basic metabolic assays, such as glucose and insulin tolerance tests. The Core will also provide consultation on the proper approach to generating recombinant and inbred strains of transgenic and knockout mice, and interact with the Genomics Core to help investigators design appropriate genotyping strategies for genome scans. In addition, the Core is in the process of developing expertise in performing glucose clamp studies to investigate whole body and organ-specific metabolic parameters. Through collaborative efforts with the other Cores of the proposed DERC, the effects of defined genetic alterations in rodents can be thoroughly characterized for their effects on metabolism, ingestive behavior, energy balance, body composition, and endocrine function. The Core will serve an important training and education function for many DERC investigators by providing advice on how to prevent common mistakes in breeding programs.