Glycomics with Staudinger activated fluorogenic dyes

描述（由申请人提供）：本研究的最终目标是提供一种在各种环境中跟踪O-连接糖基化的简便方法。这将通过利用高度适应性的施陶丁格连接活化荧光染料直接标记代谢标记的O-聚糖来实现。合成的模块化性质将允许探针的物理、化学和物理特性的独立调整。双淬灭机构应提供极低的背景，便于与各种检测系统一起使用。迄今为止，还没有特异性追踪O-连接糖基化的能力。最初，该探针将与2D凝胶电泳结合使用，以提供O-糖基化的“指纹”，并与质谱技术结合使用，以提供O-连接的糖蛋白的身份。这种方法应该极大地促进理解O-连接的糖基化的影响和调节这种翻译后修饰的机制的努力。

DESCRIPTION (provided by applicant): The ultimate goal of this research is to provide a facile method for tracking O-linked glycosylation in a variety of settings. This will be accomplished via the direct labelling of metabolically labelled O-glycans utilizing a highly adaptable Staudinger-ligation acitivatable fluorogenic dye. The modular nature of synthesis will allow independent tuning of photophysical, chemical and physical characteristics of the probe. A double quench mechanism should provide exceedingly low background, facilitating use with a variety of detection systems. The ability to specifically track O-linked glycosylation has, of yet, been unavailable. Initially this probe will be used in conjunction with 2D gel electrophoresis to provide a "fingerprint" of O-glycosylation and in conjunction with mass spectrometry techniques will provide the identity of O-linked glycoproteins. Such a method should greatly facilitate efforts to understand the impact of O-linked glycosylation and the mechnasims which regulate this post-translational modification.