Glycomics with Staudinger activated fluorogenic dyes

描述（由申请人提供）：本研究的最终目标是提供一种简便的方法来跟踪各种情况下的o -链糖基化。这将通过使用高度适应性的staudinger -结扎活化荧光染料对代谢标记的o -聚糖进行直接标记来完成。合成的模块化特性将允许独立调整探针的光物理、化学和物理特性。双淬火机构应提供极低的背景，便于与各种检测系统配合使用。特异性追踪o链糖基化的能力目前还无法实现。最初，该探针将与2D凝胶电泳结合使用，以提供o -糖基化的“指纹”，并与质谱技术结合使用，以提供o -连接糖蛋白的身份。这种方法将极大地促进理解o链糖基化的影响以及调节这种翻译后修饰的机制。

DESCRIPTION (provided by applicant): The ultimate goal of this research is to provide a facile method for tracking O-linked glycosylation in a variety of settings. This will be accomplished via the direct labelling of metabolically labelled O-glycans utilizing a highly adaptable Staudinger-ligation acitivatable fluorogenic dye. The modular nature of synthesis will allow independent tuning of photophysical, chemical and physical characteristics of the probe. A double quench mechanism should provide exceedingly low background, facilitating use with a variety of detection systems. The ability to specifically track O-linked glycosylation has, of yet, been unavailable. Initially this probe will be used in conjunction with 2D gel electrophoresis to provide a "fingerprint" of O-glycosylation and in conjunction with mass spectrometry techniques will provide the identity of O-linked glycoproteins. Such a method should greatly facilitate efforts to understand the impact of O-linked glycosylation and the mechnasims which regulate this post-translational modification.