Glycomics with Staudinger activated fluorogenic dyes
Glycomics with Staudinger activated fluorogenic dyes
批准号:
6693524
负责人:
ISAAC S CARRICO
金额:
$3.97万
依托单位国家:
美国
项目类别:
财政年份:
2003
资助国家:
美国
项目状态:
已结题
起止时间:
2003-09-15 至 2005-09-14
中文摘要
描述(由申请人提供):本研究的最终目标是提供一种简便的方法来跟踪各种情况下的o -链糖基化。这将通过使用高度适应性的staudinger -结扎活化荧光染料对代谢标记的o -聚糖进行直接标记来完成。合成的模块化特性将允许独立调整探针的光物理、化学和物理特性。双淬火机构应提供极低的背景,便于与各种检测系统配合使用。特异性追踪o链糖基化的能力目前还无法实现。最初,该探针将与2D凝胶电泳结合使用,以提供o -糖基化的“指纹”,并与质谱技术结合使用,以提供o -连接糖蛋白的身份。这种方法将极大地促进理解o链糖基化的影响以及调节这种翻译后修饰的机制。
英文摘要
DESCRIPTION (provided by applicant): The ultimate goal of this research is to provide a facile method for tracking O-linked glycosylation in a variety of settings. This will be accomplished via the direct labelling of metabolically labelled O-glycans utilizing a highly adaptable Staudinger-ligation acitivatable fluorogenic dye. The modular nature of synthesis will allow independent tuning of photophysical, chemical and physical characteristics of the probe. A double quench mechanism should provide exceedingly low background, facilitating use with a variety of detection systems. The ability to specifically track O-linked glycosylation has, of yet, been unavailable. Initially this probe will be used in conjunction with 2D gel electrophoresis to provide a "fingerprint" of O-glycosylation and in conjunction with mass spectrometry techniques will provide the identity of O-linked glycoproteins. Such a method should greatly facilitate efforts to understand the impact of O-linked glycosylation and the mechnasims which regulate this post-translational modification.
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Highly selective radioisotope modification to enable adenovirus tracking in vivo
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批准号:8772814
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项目类别:
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资助金额:$20.29万
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财政年份:2014
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负责人:ISAAC S CARRICO
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依托单位:
Glycomics with Staudinger activated fluorogenic dyes
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批准号:7147091
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项目类别:
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资助金额:$4.13万
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财政年份:2003
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负责人:ISAAC S CARRICO
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依托单位:
Glycomics with Staudinger activated fluorogenic dyes
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批准号:6827808
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项目类别:
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资助金额:$4.3万
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财政年份:2003
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负责人:ISAAC S CARRICO
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依托单位: