The Function of Cdk1/cyclin B in Mitotic Entry.

Cdk1/cyclin B 在有丝分裂进入中的功能。

• 批准号: 6585099
• 负责人: SUSANNE M STEGGERDA
• 金额: $ 4.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6585099
• 关键词: Xenopus oocyte; cell cycle; cell growth regulation; chemical genetics; cyclin dependent kinase; cyclins; cytogenetics; enzyme activity; enzyme substrate; gel electrophoresis; immunoprecipitation; mass spectrometry; molecular biology information system; phosphorylation; postdoctoral investigator; protein localization; protein structure function; radiotracer

DESCRIPTION (provided by applicant): Uncontrolled cell division is a hallmark of cancer. Although much progress has been made in deciphering the molecular basis of tumorigenesis, a deeper understanding of the complexities of cell cycle progression will be necessary for the development of novel therapeutic strategies. The transition from G2 to M phase is a highly regulated step in the cell division cycle, and as such, some proteins involved in this process are targeted in human cancers. Thus, basic research on the mechanism of mitotic entry is an important part of cancer research. The goals of this research are: 1) to define the contribution of Cdkl/cyclin B towards mitotic entry, and 2) to identify and characterize novel substrates of Cdkl/cyclin B that drive the transition from G2 phase to M phase of the vertebrate cell cycle. A chemical-genetic screen will be used to identify a comprehensive set of protein substrates for the Cdkl/cyclin B complex. Substrates will be characterized for their roles in cell cycle progression using a combination of cell biological and biochemical methods. Results from these studies will contribute toward a more detailed understanding of the events of early mitosis, including how the rise of Cdkl/cyclin B activity during late G2 phase ultimately drives entry into mitosis.

描述（由申请人提供）：