Septum Asymmetry as a Cell Fate Determinant

隔膜不对称作为细胞命运的决定因素

• 批准号: 6626191
• 负责人: ERIC C BECKER
• 金额: $ 4.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626191
• 关键词: Bacillus subtilis; antiseptic sterilization; bacterial genetics; bacterial proteins; cell differentiation; cellular polarity; chromosome movement; developmental genetics; fluorescence resonance energy transfer; food sanitation; gene environment interaction; gene mutation; intermolecular interaction; membrane proteins; microorganism reproduction; microscopy; polymerase chain reaction; postdoctoral investigator; protein localization; protein structure function; reporter genes; spores; transcription factor

DESCRIPTION (provided by applicant): The overall objective of the proposed research is to characterize the molecular events that establish cell differentiation during spore formation in Bacillus subtilis. Bacterial spores are highly resistant to many common sterilization techniques and often are implicated in food-borne illness. Understanding the process of sporulation should allow for the development of new, more effective sterilization techniques. The proposed experiments are conceptually based on a model where the developmental pathway leading to spore formation is established, in part, by the asymmetric distribution of integral membrane proteins across the sporulation septum. This model will be tested by methods designed to determine both the distribution of proteins across the septum, and the mechanisms by which these proteins are restricted to one or the other cell following division. These methods are broadly applicable to the study of other disciplines and therefore, should contribute generally to science and ultimately to human health.

简介(由申请人提供)：建议的整体目标 研究是描述建立细胞的分子事件的特征 枯草芽孢杆菌芽胞形成过程中的分化。细菌孢子 对许多常见的消毒技术具有高度的抵抗力，而且通常是 与食源性疾病有关。了解产孢子的过程 应允许开发新的、更有效的绝育方法 技巧。拟议的实验在概念上基于一个模型，在该模型中 部分地建立了导致孢子形成的发育途径， 由于整膜蛋白在整个细胞膜上的不对称分布 孢子形成隔膜。该模型将通过设计用于确定 无论是蛋白质在隔膜上的分布，还是通过 这些蛋白质被限制在下面的一个或另一个细胞上 组织。这些方法广泛适用于研究其他 因此，应该普遍地为科学和 最终影响到人类健康。