Investigation into polymer lipid particles containing G-protein coupled receptors as a tool to drug discovery and drug detection

基于G蛋白偶联受体（GPCR）的药物发现和开发可以通过有效使用聚合物脂质颗粒（PoLiPa）来简化。该项目的目的是研究可用于制备PoLiPa的新聚合物，以制备纯化和稳定的G蛋白偶联受体GPCR。在产生含有GPCR的PoLiPas后，许多对于确定GPCR结构、结合性质和生物物理特性重要的技术成为可能。这包括表面等离子体共振（SPR）;低温电子显微镜（cryo-EM）;微尺度热泳（MST）;质谱（MS）和动态光散射（DLS）。该项目的预测结果是评估PoLiPa的不同替代聚合物对3-4种不同技术的有效性，这些技术对3种不同GPCR的药物发现很重要。这将允许更有效地使用PoliPas来测试更广泛的GPCR。在不同的PoliPas上测试这些技术可以提供关于GPCR与其他蛋白质、脂质和分子相互作用的关键信息，这些信息可能有助于开发新药。

Drug discovery and development based on G-protein coupled receptors (GPCRs) could be streamlined by efficient use of polymer lipid particles (PoLiPa). The aim of this project is to investigate new polymers that can be used to make PoLiPa in order to prepare purified and stable G-protein coupled receptors GPCRs. Many techniques important for determining GPCR structure, binding properties and biophysical characteristics become possible after producing PoLiPas containing GPCRs. This includes surface plasmon resonance (SPR); cryogenic electron microscopy (cryo-EM); microscale thermophoresis (MST); Mass Spectrometry (MS) and Dynamic Light scattering (DLS). The predicted outcome of the project is assessment of the effectiveness of different alternative polymers to PoLiPa on 3-4 different techniques important in drug discovery for 3 different GPCRs. This will allow more effective use of PoliPas for testing a wider range of GPCRs. Testing the techniques on different PoliPas may provide key information on GPCRs interact with other proteins, lipids and molecules which could be useful in developing a new drug.