PROTEIN IDENTIFICATION CORE

蛋白质鉴定核心

• 批准号: 6846687
• 负责人: KATHRYN STONE
• 金额: $ 16.73万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6846687
• 关键词: biomarker; biomedical facility; drug addiction; high performance liquid chromatography; ion cyclotron resonance spectrometry; lipids; liquid chromatography mass spectrometry; mass spectrometry; matrix assisted laser desorption ionization; nerve /myelin protein; neurotoxicology; posttranslational modifications; protein quantitation /detection; protein sequence; protein structure function; proteomics

The overall goal of the Protein Identification Core, which will be located within the MS Resource of the Keck Laboratory, will be to identify proteins and their post-translational modifications that are of interest to members of the Neuroproteomics Center and that have been isolated either by individual Center members or by the Protein and Lipid Separation and Profiling Core. In the latter instance, the proteins or peptides of interest may have been determined to be differentially expressed in response to a stimulus or disease based on differential (fluorescence) gel electrophoresis (DIGE) (1), the Beckman-Coulter ProteomeLab PF 2D liquid separation approach (2), or MALDI-MS based biomarker discovery using the algorithm developed by staff in the proposed Bioinformatics and Biostatistics Core (3). In addition to providing existing protein identification technologies to members of the NIDA Neuroproteomies Center, the Protein Identification Core also proposes to extend and improve these technologies.

蛋白质鉴定核心将位于凯克实验室的 MS 资源内，其总体目标是鉴定神经蛋白质组中心成员感兴趣的蛋白质及其翻译后修饰，并且这些蛋白质及其翻译后修饰已由个别中心成员或蛋白质和脂质分离和分析核心分离。在后一种情况下，基于差异（荧光）凝胶电泳 (DIGE) (1)、Beckman-Coulter ProteomeLab PF 2D 液体分离方法 (2) 或基于 MALDI-MS 的生物标志物发现，可能已确定感兴趣的蛋白质或肽在对刺激或疾病的反应中差异表达，这些算法由生物信息学和生物信息学领域的工作人员开发。 生物统计学核心（3）。除了向NIDA神经蛋白质组中心成员提供现有的蛋白质鉴定技术外，蛋白质鉴定核心还建议扩展和改进这些技术。