PROTEIN IDENTIFICATION CORE

蛋白质鉴定核心

• 批准号: 7076275
• 负责人: KATHRYN STONE
• 金额: $ 17.24万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7076275
• 关键词: biomarker; biomedical facility; drug addiction; high performance liquid chromatography; ion cyclotron resonance spectrometry; lipids; liquid chromatography mass spectrometry; mass spectrometry; matrix assisted laser desorption ionization; nerve /myelin protein; neurotoxicology; posttranslational modifications; protein quantitation /detection; protein sequence; protein structure function; proteomics

The overall goal of the Protein Identification Core, which will be located within the MS Resource of the Keck Laboratory, will be to identify proteins and their post-translational modifications that are of interest to members of the Neuroproteomics Center and that have been isolated either by individual Center members or by the Protein and Lipid Separation and Profiling Core. In the latter instance, the proteins or peptides of interest may have been determined to be differentially expressed in response to a stimulus or disease based on differential (fluorescence) gel electrophoresis (DIGE) (1), the Beckman-Coulter ProteomeLab PF 2D liquid separation approach (2), or MALDI-MS based biomarker discovery using the algorithm developed by staff in the proposed Bioinformatics and Biostatistics Core (3). In addition to providing existing protein identification technologies to members of the NIDA Neuroproteomies Center, the Protein Identification Core also proposes to extend and improve these technologies.

蛋白质鉴定核心的总体目标将位于凯克实验室的MS资源内，将鉴定神经蛋白质组学中心成员感兴趣的蛋白质及其翻译后修饰，并且这些蛋白质已被中心成员或蛋白质和脂质分离和分析核心分离。在后一种情况下，基于差异（荧光）凝胶电泳（DIGE）（1）、Beckman-Coulter ProteomeLab PF 2D液体分离方法（2）或基于MALDI-MS的生物标志物发现（使用由工作人员在拟议的生物信息学和生物统计学核心（3）中开发的算法），可以确定目标蛋白或肽在响应刺激或疾病时差异表达。除了向NIDA神经蛋白质组学中心的成员提供现有的蛋白质鉴定技术外，蛋白质鉴定核心还建议扩展和改进这些技术。