Otitis Media Core Center

中耳炎核心中心

• 批准号: 6791386
• 负责人: KATHLEEN Ann DALY
• 金额: $ 42.36万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-24 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6791386
• 关键词: biomedical facility; otitis media

DESCRIPTION (provided by applicant): Otitis media is among the most common childhood diseases, and otitis media sequelae are frequent in adults. A multidisciplinary team of basic and clinical scientists has led otitis media research at the University of Minnesota since 1978, when the University's Otitis Media Research Center (OMRC) was formed. The OMRC mission is to create a collaborative environment for interdisciplinary research that stimulates advancement in understanding otitis media pathogenesis, to inspire new developments in diagnostic technology, treatment and prevention modalities, and to generate and disseminate fundamental and clinical knowledge, thereby, enhancing patient care. The Otitis Media Core Center will support 16 currently NIH-funded base research projects (12 NIDCD-funded) and an additional two recently submitted projects. OMRC research is focused in basic molecular, cellular, microbial, immunological, animal modeling, population science, and clinical studies. Research productivity is demonstrated in the more than 400 peer-reviewed publications from OMRC faculty. Most of the proposed Core Laboratories were developed under NIDCD P01 support from 1978 to 1996. The Analytical Pathobiology Core will continue to provide expertise and techniques in microbiology, immunology, biochemistry, molecular biology, and histopathology. It will also provide antibodies, probes, archival samples, bacteria or bacterial components, and a complete range of histological and molecular biological services on human and animal temporal bones. Services include light and electron microscopy, histochemistry, immunohistochemistry, immunogold, quantitative image analysis, Northern blot, RNA protection assay, Western blot, in situ hybridization, competitive RT-PCR, representative differential display, and molecular expression and cloning. Immunological expertise includes immunoassay development, immunological reagent production, molecular characterization of a pneumococcal library used in otitis media animal modeling, and refinement of animal modeling surgical techniques. The Human Subjects & Biostatistics Core will continue to be responsible for assisting base project PIs in study design, data collection and processing, quality control, data storage and retrieval, and data analysis of all studies to provide all investigators with a high level and broad range of expertise in data management and analysis. A unique feature of the Core will be the provision of pilot data for new projects drawing from databases and participants from previous OM studies. The key areas are quality control and standardization of data analyses. The key areas are in both Cores are quality assurance, assay standardization, and utilization of the most sensitive methodologies available. Together, the Cores will greatly enhance the OMRC infrastructure, allowing highly productive basic and clinical scientists to conduct research on a broad range of middle and inner ear disorders.

描述（由申请人提供）： 中耳炎是最常见的疾病之一 儿童疾病和中耳炎后遗症在成人中很常见。一个 由基础和临床科学家组成的多学科团队领导了中耳炎 自 1978 年起在明尼苏达大学进行研究，当时该大学的中耳炎 媒体研究中心（OMRC）成立。 OMRC 的使命是创建一个 促进跨学科研究的协作环境 对中耳炎发病机制的了解取得进展，以激发新的灵感 诊断技术、治疗和预防方式的发展，以及 产生和传播基础和临床知识，从而， 加强患者护理。 Otitis Media Core Center 目前将支持 16 个 NIH 资助的基础研究项目（12 个 NIDCD 资助）和另外两个 最近提交的项目。 OMRC 研究重点是基础分子、 细胞、微生物、免疫学、动物模型、人口科学等 临床研究。研究生产力在 400 多个 OMRC 教师的同行评审出版物。大多数建议的核心 实验室是在 1978 年至 1996 年间在 NIDCD P01 支持下开发的。 分析病理学核心将继续提供专业知识和技术 微生物学、免疫学、生物化学、分子生物学等 组织病理学。它还将提供抗体、探针、档案样本、 细菌或细菌成分，以及完整的组织学和 人类和动物颞骨的分子生物学服务。服务 包括光学和电子显微镜、组织化学、免疫组织化学、 免疫金、定量图像分析、Northern blot、RNA 保护测定、 蛋白质印迹、原位杂交、竞争性 RT-PCR、代表性 差异显示、分子表达和克隆。免疫学 专业知识包括免疫测定开发、免疫试剂生产、 中耳炎动物肺炎球菌文库的分子特征 建模和动物建模手术技术的完善。人类 科目和生物统计核心将继续负责协助基地 研究设计、数据收集和处理、质量控制等方面的项目 PI 数据存储和检索，以及所有研究的数据分析，以提供所有 在数据管理方面具有高水平和广泛专业知识的研究人员 和分析。核心的一个独特功能是提供试点数据 用于从数据库和以前 OM 的参与者中提取的新项目 研究。关键领域是质量控制和数据分析标准化。 两个核心的关键领域是质量保证、测定标准化、 并利用可用的最敏感的方法。在一起， 核心将极大地增强 OMRC 基础设施，从而实现高生产力 基础和临床科学家对广泛的中间和领域进行研究 内耳疾病。