Otitis Media Core Center

描述(申请人提供)：中耳炎是最常见的 儿童疾病和中耳炎后遗症在成人中很常见。一个 由基础和临床科学家组成的多学科团队领导了中耳炎 自1978年以来在明尼苏达大学的研究，当时该大学的耳炎 媒体研究中心(OMRC)成立。OMRC的任务是创建一个 促进跨学科研究的协作环境 认识中耳炎发病机制的进展，启发新的 诊断技术、治疗和预防模式的发展；以及 产生和传播基础和临床知识，从而， 加强对病人的护理。奥蒂斯媒体核心中心目前将支持16个 NIH资助的基础研究项目(12个NIDCD资助)和另外两个 最近提交的项目。OMRC的研究主要集中在基础分子， 细胞、微生物、免疫学、动物模型、种群科学和 临床研究。研究效率在400多个项目中得到证明 OMRC教职员工的同行评议出版物。大部分建议的核心 1978年至1996年，在NIDCD P01的支持下发展了实验室。这个 分析病理生物学核心将继续提供专业知识和技术 在微生物学、免疫学、生物化学、分子生物学和 组织病理学。它还将提供抗体，探针，档案样本， 细菌或细菌成分，以及完整的组织学和 为人类和动物的颞骨提供分子生物学服务。服务 包括光学和电子显微镜、组织化学、免疫组织化学 免疫金，定量图像分析，Northern印迹，RNA保护试验， 蛋白质印迹、原位杂交、竞争性RT-PCR、代表性 差异显示，以及分子表达和克隆。免疫学 专业知识包括免疫分析开发、免疫试剂生产、 用于中耳炎动物的肺炎链球菌文库的分子特征 动物模型外科技术的建模和改进。《人类》 学科和生物统计核心将继续负责协助基地 研究设计、数据收集和处理、质量控制、 数据存储和检索，以及所有研究的数据分析，以提供所有 在数据管理方面具有高水平和广泛专业知识的调查人员 和分析。核心的一个独特特点将是提供试点数据 对于从数据库绘制的新项目和来自以前OM的参与者 学习。关键领域是质量控制和数据分析的标准化。 关键领域在两个核心都是质量保证，检测标准化， 以及利用现有的最敏感的方法。团结在一起， 内核将极大地增强OMRC基础设施，从而实现高效 基础和临床科学家对广泛的中间和中间和 内耳失调。

DESCRIPTION (provided by applicant): Otitis media is among the most common childhood diseases, and otitis media sequelae are frequent in adults. A multidisciplinary team of basic and clinical scientists has led otitis media research at the University of Minnesota since 1978, when the University's Otitis Media Research Center (OMRC) was formed. The OMRC mission is to create a collaborative environment for interdisciplinary research that stimulates advancement in understanding otitis media pathogenesis, to inspire new developments in diagnostic technology, treatment and prevention modalities, and to generate and disseminate fundamental and clinical knowledge, thereby, enhancing patient care. The Otitis Media Core Center will support 16 currently NIH-funded base research projects (12 NIDCD-funded) and an additional two recently submitted projects. OMRC research is focused in basic molecular, cellular, microbial, immunological, animal modeling, population science, and clinical studies. Research productivity is demonstrated in the more than 400 peer-reviewed publications from OMRC faculty. Most of the proposed Core Laboratories were developed under NIDCD P01 support from 1978 to 1996. The Analytical Pathobiology Core will continue to provide expertise and techniques in microbiology, immunology, biochemistry, molecular biology, and histopathology. It will also provide antibodies, probes, archival samples, bacteria or bacterial components, and a complete range of histological and molecular biological services on human and animal temporal bones. Services include light and electron microscopy, histochemistry, immunohistochemistry, immunogold, quantitative image analysis, Northern blot, RNA protection assay, Western blot, in situ hybridization, competitive RT-PCR, representative differential display, and molecular expression and cloning. Immunological expertise includes immunoassay development, immunological reagent production, molecular characterization of a pneumococcal library used in otitis media animal modeling, and refinement of animal modeling surgical techniques. The Human Subjects & Biostatistics Core will continue to be responsible for assisting base project PIs in study design, data collection and processing, quality control, data storage and retrieval, and data analysis of all studies to provide all investigators with a high level and broad range of expertise in data management and analysis. A unique feature of the Core will be the provision of pilot data for new projects drawing from databases and participants from previous OM studies. The key areas are quality control and standardization of data analyses. The key areas are in both Cores are quality assurance, assay standardization, and utilization of the most sensitive methodologies available. Together, the Cores will greatly enhance the OMRC infrastructure, allowing highly productive basic and clinical scientists to conduct research on a broad range of middle and inner ear disorders.