Establishing Chlamydomonas reinhardtii as an industrial chassis for the production of high value biochemicals

建立莱茵衣藻作为生产高价值生化产品的工业基础

• 批准号: 2435955
• 金额: --
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2435955
• 关键词: Establishing; Chlamydomonas; reinhardtii; industrial; chassis

The green alga Chlamydomonas reinhardtii is an invaluable reference organism to research fields including algal, plant and ciliary biology (1). The plethora of genetic and molecular techniques available for its manipulation means it is now at the cusp of becoming a photoautotrophic industrial biotechnology (IB) platform on par with Escherichia coli or yeast (2). However, long-standing inefficiencies in transgene expression and targeted nuclear gene editing have been remaining hurdles to this potential being fully exploited. Recent technological advances including our seminal work using CRISPR (3) enabled efficient, targeted gene modifications with nucleotide precision in C. reinhardtii. These transformational advances now enable, for the first time, rapid engineering of C. reinhardtii as photoautotrophic strains for IB. Remaining limitations for effective metabolic engineering include transgene silencing and instability. However, gene editing now allows us to overcome these limitations via rapid strain engineering. This project aims to develop C. reinhardtii as an industrial chassis through extensive gene editing efforts in close collaboration with our industrial partner, ScotBio (www.scotbio.com).

该绿色衣藻莱茵衣藻是一个宝贵的参考生物研究领域，包括藻类，植物和纤毛生物学（1）。大量的遗传和分子技术可用于其操作，这意味着它现在正处于成为与大肠杆菌或酵母相当的光自养工业生物技术（IB）平台的风口浪尖（2）。然而，转基因表达和靶向核基因编辑的长期低效一直是充分利用这一潜力的障碍。最近的技术进步，包括我们使用CRISPR（3）的开创性工作，使C.莱因哈德氏菌这些变革性的进展现在第一次使C。reinhardtii作为IB的光合自养菌株。有效代谢工程的剩余限制包括转基因沉默和不稳定性。然而，基因编辑现在允许我们通过快速应变工程来克服这些限制。本项目旨在开发C.通过与我们的工业合作伙伴ScotBio（www.scotbio.com）密切合作，通过广泛的基因编辑工作，将莱茵衣藻作为工业底盘。