Tracking Intracellular Transport of Self-Reporting Nanomaterials

合成大分子和天然大分子的运输过程尚不完全清楚，但它决定着药物是否在正确的地点、正确的时间和正确的剂量被递送。该项目将着眼于新的合成和天然大分子以及实时报告递送的探针。我们将在荧光标记的大分子及其环境的选定“地标”之间使用FRET和相关的光学接近探针，以获得分子水平上生物环境（组织和细胞）定位的信息。该项目将包括新的反应性和可生物降解的聚合物传递系统的合成，荧光探针的结合，传递系统的评估和细胞/组织培养中的货物运输，以及潜在的体内实验。最后，我们的目标是举例说明新的材料和知识的贩运，以提高siRNA敲低在急性肾损伤（AKI）中的治疗效果。

CDT Theme alignment: Advanced Product Design Transport processes for synthetic and natural macromolecules are not fully understood, but govern whether a drug is delivered in the right place, at the right time, and the right dose. This project will look at new synthetic and natural macromolecules and probes for real-time reporting of delivery. We will use FRET and related optical proximity probes between fluorescently labelled macromolecules and selected 'landmarks' of their environment, to gain information on localization in biological settings (tissue and cell) at the molecular level. The project will encompass synthesis of new responsive and biodegradable polymer delivery systems, conjugation of fluorescent probes, evaluation of delivery system and cargo transport in cell / tissue culture, and potentially in vivo experiments. Finally, we aim to exemplify the new materials and knowledge of trafficking to enhance therapeutic efficacy of siRNA knockdown in acute kidney injury (AKI)