ANALGESIC REGIMENS FOR SURGERY AND INFLAMMATION IN MICE

描述(由申请人提供)：识别和减轻疼痛是实验动物医学中常见的挑战，由《动物福利法》和《关于使用实验动物的公共卫生服务政策》规定。然而，有关啮齿动物手术后有效止痛的现有数据主要是通过研究大鼠收集的，相对较少的研究专门评估了小鼠的临床止痛效果。小鼠兽医管理的临床方法的发展落后于转基因小鼠品系的可获得性和科学使用的爆炸性增长，特别是在需要外科操作的研究中它们日益重要的方面。如果美国农业部最终修改“动物”的定义，将小鼠包括在内，那么为小鼠确定有效的止痛方案的必要性将变得更加迫切。 本申请建议评估几种口服止痛方案对小鼠急性、外科和炎症性疼痛的疗效。通过饮用水口服止痛药在研究环境中是有利的，因为它允许有效地治疗大量动物，而不需要额外的操作。然而，重要的考虑因素是确保治疗方案对动物正在经历的疼痛有效，以及动物自愿消耗足够的药物以达到有效剂量。现有的许多文献都没有涉及这些问题。初步工作表明，在饮用水中给予布洛芬可以促进接受腹部手术的小鼠的行为恢复。这项申请建议通过确定其他或更好的止痛方案来扩大这项工作，以治疗小鼠的急性、外科或炎症性疼痛。这些数据应该会揭示缓解小鼠术后疼痛的新的实用策略。

DESCRIPTION (provided by applicant): Recognizing and alleviating pain are common challenges in laboratory animal medicine and are mandated by the Animal Welfare Act and the Public Health Service Policy on the Use of Laboratory Animals. However, available data on effective post-surgical analgesia for rodents has been collected primarily by studying rats; relatively few studies specifically assess analgesic efficacy for clinical use in mice. The development of clinical approaches for veterinary medical management of mice has lagged behind the explosive expansion in the availability and scientific use of genetically modified strains of mice, particularly with respect to their increasing importance in studies requiring surgical manipulation. The need to identify efficacious analgesic regimens for mice will become even more imperative should the United States Department of Agriculture eventually amend its definition of "animal" to include the genus Mus. This application proposes to evaluate the efficacy of several oral analgesic regimens for treatment of acute, surgical, and inflammatory pain in mice. Oral administration of analgesic medications via the drinking water is advantageous in a research setting because it permits large numbers of animals to be treated efficiently and without additional manipulation. However, important considerations are to assure both that the therapeutic regimen has efficacy against the pain the animals are experiencing and that the animals voluntarily consume enough medication to achieve effective doses. Much of the available literature does not address these issues. Preliminary work indicates that ibuprofen administered in the drinking water promotes behavioral recuperation in mice that have undergone abdominal surgery. This application proposes to extend that work by identifying additional or superior analgesic regimens for acute, surgical or inflammatory pain in mice. The data should reveal new and practical strategies for relief of post-procedural pain in mice.