Accelerating disease resistance breeding for diploid potato germplasm improvement

加快马铃薯二倍体种质改良抗病育种

• 批准号: 2436905
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2436905
• 关键词: Accelerating; disease; resistance; breeding; diploid

Pests and diseases reduce crop yields, requiring agrichemical sprays. We aim to replace agrichemicals with genetic resistance for pest and disease control. This project involves novel approaches (https://tinyurl.com/ycn4od6f) to understand and use potato diversity for disease resistance using newly available self-compatible diploid potato lines. Tetraploid potato lines carry useful variation for pest and disease resistance, but genetic analysis in tetraploids is difficult. Using appropriate crosses, diploid genetics will be used to define, introgress to diploids, and clone potato leaf roll virus (PLRV) and nematode resistance genes, and will use genome editing to accelerate breeding such genes into useful diploids and improve potato traits. CRISPR/Cas9 editing will be used for diploid potato improvement.

虫害和疾病降低了作物产量，需要进行农化喷洒。我们的目标是取代具有遗传抗性的农用化学品，用于病虫害控制。该项目涉及新的方法(https://tinyurl.com/ycn4od6f))，以了解和利用马铃薯多样性来利用新获得的自交亲和二倍体马铃薯品系的抗病能力。四倍体马铃薯品系在病虫害抗性方面具有有用的变异，但四倍体的遗传分析是困难的。利用合适的杂交组合，二倍体遗传学将被用于定义、导入和克隆马铃薯卷叶病毒(PLRV)和线虫抗性基因，并将利用基因组编辑加速将这些基因培育成有用的二倍体并改良马铃薯性状。CRISPR/Cas9编辑将用于二倍体马铃薯的改良。