Accelerating disease resistance breeding for diploid potato germplasm improvement

病虫害降低作物产量，需要喷洒农药。我们的目标是用遗传抗性代替农药防治病虫害。该项目涉及新方法（https://tinyurl.com/ycn4od6f），利用新获得的自相容二倍体马铃薯品系来了解和利用马铃薯的多样性以抵抗疾病。四倍体马铃薯株系携带有用的抗病虫害变异，但对四倍体进行遗传分析是困难的。利用合适的杂交，二倍体遗传学将被用于定义、向二倍体渗透，并克隆马铃薯卷叶病毒（PLRV）和线虫抗性基因，并将使用基因组编辑加速将这些基因培育成有用的二倍体并改善马铃薯性状。CRISPR/Cas9编辑将用于二倍体马铃薯的改良。

Pests and diseases reduce crop yields, requiring agrichemical sprays. We aim to replace agrichemicals with genetic resistance for pest and disease control. This project involves novel approaches (https://tinyurl.com/ycn4od6f) to understand and use potato diversity for disease resistance using newly available self-compatible diploid potato lines. Tetraploid potato lines carry useful variation for pest and disease resistance, but genetic analysis in tetraploids is difficult. Using appropriate crosses, diploid genetics will be used to define, introgress to diploids, and clone potato leaf roll virus (PLRV) and nematode resistance genes, and will use genome editing to accelerate breeding such genes into useful diploids and improve potato traits. CRISPR/Cas9 editing will be used for diploid potato improvement.