The role of cryopyrin in autoinflammatory diseases

隐热蛋白在自身炎症性疾病中的作用

• 批准号: 6937116
• 负责人: HAROLD M HOFFMAN
• 金额: $ 30.4万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6937116
• 关键词: autoimmune disorder; blood proteins; clinical research; cold temperature; family genetics; gene mutation; human subject; inflammation; molecular pathology; nuclear factor kappa beta; protein protein interaction; protein structure function

DESCRIPTION (provided by applicant): Familial cold autoinflammatory syndrome (FCAS) is an example of the immune system's response to physical stimuli. This application seeks to broaden our knowledge of the molecular mechanisms of inflammation, using FCAS as a model. This disease provides a unique opportunity to study inflammation because we recently identified the gene (CIAS1) and protein (cryopyrin) responsible for FCAS. Many highly motivated patients are available for participation, and FCAS inflammatory symptoms can be elicited and measured in a controlled environment (cold challenge). As in FCAS, frequently there are few safe and effective treatments for many of the inflammatory disorders, so our research which may ultimately lead to novel therapeutics for inflammatory disorders is important clinically as well as scientifically. The primary objective of this project is to elucidate the normal and abnormal structure and function of cryopyrin in order to develop a better understanding of the physiologic basis of FCAS and other inflammatory diseases. These aims will be accomplished utilizing established molecular genetics techniques, new and established protocols for generation of antibodies, and advanced imaging techniques. The specific aims of this application are (1) further investigation of the genetics of FCAS including identification of additional mutations and correlation of these mutations with clinical features, (2) delineation of the CIAS1 gene expression in tissues, leukocytes, and cell lines (3) characterization of the cryopyrin protein structure, localization, and expression and initial studies of the function of cryopyrin by evaluating signaling pathways and proteins with which it interacts, and (4) determination of the mechanisms of inflammation that occur with an experimental cold room challenge in these patients by observation and collection of clinical samples. The achievement of these goals will improve our understanding of inflammation in FCAS as well as other inflammatory disorders.

描述（由申请人提供）：家族性感冒自身炎症综合征（FCAS）是免疫系统对物理刺激反应的一个例子。该应用程序旨在扩大我们对炎症分子机制的认识，使用FCAS作为模型。这种疾病为研究炎症提供了一个独特的机会，因为我们最近发现了负责FCAS的基因（CIAS1）和蛋白质（cryopyrin）。许多积极性很高的患者可以参与，FCAS炎症症状可以在受控环境（冷挑战）中引起和测量。与FCAS一样，许多炎症性疾病往往缺乏安全有效的治疗方法，因此我们的研究可能最终导致炎症性疾病的新治疗方法，这在临床上和科学上都很重要。本项目的主要目的是阐明crypyrin的正常和异常结构和功能，以便更好地了解FCAS和其他炎症性疾病的生理基础。这些目标将利用已建立的分子遗传学技术、新的和已建立的抗体生成方案以及先进的成像技术来实现。