Molecular Biology of Drosophila Learning and Memory

果蝇学习和记忆的分子生物学

• 批准号: 6771137
• 负责人: Ronald L Davis
• 金额: $ 43.79万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-12-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6771137
• 关键词: Drosophilidae; behavioral /social science research tag; behavioral genetics; cell adhesion molecules; focal adhesion kinase; gene expression; gene mutation; genetically modified animals; integrins; learning; long term memory; memory; molecular biology; olfactions; operant conditionings; transcription factor

We propose experiments designed to increase our understanding of the roles for certain types of cell adhesion molecules in learning and memory. Prior research has indicated that certain members of the integrin family of cell adhesion molecules, and members of the immunoglobulin superfamily of cell adhesion molecules, are important for odor learning in Drosophila. Mutants that are defective in the expression of these molecules will be studied molecularly and behaviorally. Some experiments will determine whether expression of transgenes in restricted portions of the brain will rescue the mutant learning deficits. This will reveal whether limited spatial expression is sufficient for behavioral rescue. The mutants will also be tested in operant learning situations to determine whether the behavioral deficit is specific for odor learning, or whether the molecules have a more general influence on conditioned behavior. Over-expression of some cell adhesion molecules blocks long-term memory. Experiments are proposed to define the time window of over-expression necessary to observe the block. And, we will pursue an understanding of how these cell adhesion molecules mediate behavior by studying molecules known to organize adhesion molecules at the synapse, and candidate signaling molecules known to function downstream of the cell adhesion receptors. These experiments will further our understanding of the molecular and cellular basis of learning and memory in Drosophila. This basic information is expected to be of general significance to learning in other species and of relevance to diseases that affect cognition.

我们提出的实验，旨在增加我们的理解，某些类型的细胞粘附分子在学习和记忆中的作用。 先前的研究已经表明，细胞粘附分子的整合素家族的某些成员，以及细胞粘附分子的免疫球蛋白超家族的成员，对果蝇的气味学习很重要。 这些分子表达缺陷的突变体将在分子和行为上进行研究。一些实验将确定转基因在大脑限制部分的表达是否会挽救突变的学习缺陷。 这将揭示有限的空间表达是否足以进行行为拯救。 突变体还将在操作性学习情况下进行测试，以确定行为缺陷是否是气味学习特有的，或者这些分子是否对条件行为具有更普遍的影响。 某些细胞粘附分子的过度表达会阻断长期记忆。 实验提出了定义的时间窗口观察块所需的过度表达。 并且，我们将通过研究已知在突触处组织粘附分子的分子以及已知在细胞粘附受体下游起作用的候选信号分子来理解这些细胞粘附分子如何介导行为。 这些实验将进一步加深我们对果蝇学习和记忆的分子和细胞基础的理解。 这一基本信息预计将对其他物种的学习具有普遍意义，并与影响认知的疾病有关。