Plasticity of Vocal Control
声音控制的可塑性
基本信息
- 批准号:6834627
- 负责人:
- 金额:$ 11.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-01-01 至 2007-12-31
- 项目状态:已结题
- 来源:
- 关键词:animal communication behaviorbehavioral /social science research taggene expressiongene targetinggenetic mappinghormone regulation /control mechanismin situ hybridizationlearningmolecular cloningneural plasticityneuroanatomyneuroendocrine systempolymerase chain reactionpsychobiologysongbirdssteroid hormonevocalization
项目摘要
DESCRIPTION (provided by applicant): The long-term goal of this research is to understand the neuroendocrine basis of behavior used in communication. The birdsong system is a valuable model because song is learned, and its neural circuits are sexually dimorphic, hormone sensitive, and plastic in adults. Seasonal plasticity of the song control system provides an especially interesting model of plasticity in vertebrate brains, and a continuing goal of this research program is to understand the mechanisms and functional consequences of these seasonal changes. The immediate goal of this application is to receive training in methods of molecular biology that will enable the candidate to extend his studies of plasticity of the song system to a molecular level of analysis. Brenowitz will learn gene cloning, mapping and targeting, single and double-label in situ hybridization, PCR, and nonviral cRNA transfection methods in the laboratory of Robert Steiner at the University of Washington (UW). The candidate will be trained by Paul Neiman of the Fred Hutchison Cancer Research Center in Seattle in the use of eDNA microarrays to analyze patterns of gene expression, and in bioinformatic methods for data analysis. Brenowitz will take a course on microarray analysis at the UW Center for Expression Arrays. Scott Edwards of UW will train Brenowitz in DNA sequencing methods. The candidate will "apprentice" at the Institute for Systems Biology in Seattle to learn current methods of proteomics and bioinformatics. UW has a strong research program, with particular strengths in birdsong, endocrinology, neuroscience, molecular biology, animal behavior, and hearing research. The candidate's appointments in the Depts. of Psychology, Zoology, and the Bloedet Hearing Research Center provide access to shared facilities that benefit his research program. The proposed research will address mechanisms and functional consequences of seasonal plasticity in the song system. Aims 1 & 2, will test the hypotheses that social enhancement of seasonal growth is mediated by auditory cues and involves increased neuronal recruitment. Aim 3 will test the hypothesis that afferent innervation is necessary to maintain seasonally grown song nuclei. Aim 4 will test the hypothesis that seasonal growth of song circuits is mediated by estrogenic metabolites of testosterone. Aim 5 will use operant conditioning to test the hypothesis that seasonal plasticity of the song nuclei causes seasonal changes in song perception. Aim 6 will use in situ hybridization to test the hypothesis that expression of the gene for brain derived neurotrophic factor is upregulated when the song circuits are seasonally growing. Aim 7 will use subtractive suppressive hybridization to identify genes that are enriched in a song nucleus that grows seasonally compared with a nucleus that does not grow. Aim 8 will use a customized eDNA microarray to analyze global patterns of gene expression that are associated with seasonal growth of the telencephalic song control circuits. The results of the proposed studies will increase our understanding of the influences of steroid hormones and social stimuli on the nervous system, and the relationship between plasticity in the adult brain and learning.
描述(由申请人提供):本研究的长期目标是了解交流中使用的行为的神经内分泌基础。鸟鸣系统是一个有价值的模型,因为歌声是学习的,它的神经回路是两性异形的,激素敏感的,在成年人中是可塑的。歌曲控制系统的季节性可塑性为脊椎动物大脑的可塑性提供了一个特别有趣的模型,本研究计划的持续目标是了解这些季节性变化的机制和功能后果。该应用程序的直接目标是接受分子生物学方法的培训,这将使候选人能够将其对歌曲系统可塑性的研究扩展到分子水平的分析。布雷诺维茨将在华盛顿大学(UW)罗伯特·施泰纳(Robert Steiner)的实验室学习基因克隆、定位和靶向、单标记和双标记原位杂交、PCR和非病毒cRNA转染方法。候选人将接受西雅图弗雷德·哈奇森癌症研究中心的保罗·内曼的培训,学习如何使用eDNA微阵列分析基因表达模式,以及如何使用生物信息学方法进行数据分析。布雷诺维茨将在华盛顿大学表达阵列中心学习微阵列分析课程。华盛顿大学的斯科特·爱德华兹将培训布雷诺维茨DNA测序方法。候选人将在西雅图的系统生物学研究所“学徒”,学习蛋白质组学和生物信息学的最新方法。华盛顿大学有一个强大的研究计划,在鸟鸣,内分泌学,神经科学,分子生物学,动物行为学和听力研究的特别优势。候选人在各部门的任命。心理学,动物学和布洛德特听力研究中心提供共享设施,有利于他的研究计划。这项研究将解决鸣唱系统中季节性可塑性的机制和功能后果。目的1和2,将测试的假设,社会增强的季节性增长是介导的听觉线索,并涉及增加神经元的招聘。目的3将测试的假设,传入神经支配是必要的,以维持季节性增长的歌曲核。目的4将测试的假设,即季节性增长的歌曲电路介导的雌激素代谢产物的睾酮。目的5将使用操作性条件反射来检验鸣唱核团的季节性可塑性导致鸣唱知觉的季节性变化的假设。目的6将使用原位杂交来验证这一假设,即脑源性神经营养因子的基因表达上调时,歌曲电路是季节性增长。Aim 7将使用消减抑制杂交来鉴定基因,与不生长的细胞核相比,这些基因在季节性生长的鸣唱细胞核中富集。Aim 8将使用定制的eDNA微阵列来分析与端脑歌曲控制回路的季节性增长相关的基因表达的全球模式。这些研究的结果将增加我们对类固醇激素和社会刺激对神经系统的影响以及成人大脑可塑性与学习之间关系的理解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ELIOT A BRENOWITZ其他文献
ELIOT A BRENOWITZ的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ELIOT A BRENOWITZ', 18)}}的其他基金
Mechanisms of adult forebrain neural circuit regeneration
成人前脑神经回路再生机制
- 批准号:
10112966 - 财政年份:2018
- 资助金额:
$ 11.79万 - 项目类别: