Lung Cancer Cell Selection for Flow-Optical Tomography

用于流式光学断层扫描的肺癌细胞选择

• 批准号: 6613860
• 负责人: TATYANA A ZHUKOV
• 金额: $ 14.5万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-23 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6613860
• 关键词: bioimaging /biomedical imaging; biotechnology; clinical research; densitometry; diagnosis design /evaluation; digital imaging; flow cytometry; genetic markers; high throughput technology; human subject; human tissue; immunocytochemistry; lung neoplasms; neoplasm /cancer diagnosis; neoplastic growth; respiratory epithelium; sputum; tomography

DESCRIPTION (provided by applicant): Flow Optical Tomography (FOT) is a novel technology, currently in early stage development by Vision Gate Inc., for rapidly imaging individual stained cells in a high-throughput flow stream. Multiple point projection sources and detectors generate 2D and 3D densitometric images and chromagen quantitation at subcellular resolution. In this pilot study, we build on our past experience and collaborate with Vision Gate to evaluate the promising ability of FOT through three Aims:- First, we will select from commercially available nuclear stains those most compatible with FOT technology.- Second, we will use our existing imaging technology to create and validate a digital library of pre-malignancy associated features from nuclear stained and immuno-cytochemical markers.- Finally, we will determine whether FOT can capture these 2D morphologic and densitometric features of lung cancer and pre-cancerous cells from a high throughput flow of archived sputum specimens produced by individuals with known clinical outcomes from the ongoing Moffitt lung cancer screening study.In the proposed investigation, we will evaluate the ability of FOT to capture, in a high-throughput manner, the relevant diagnostic information for lung cancer early detection using both morphology and densitometry to enable the development of a platform for mass screening. Vision Gate technology also supports this project by developing, in a separate study, a high throughput specimen-processing device. This provides specimens for the FOT that will be enriched by removal of confounding debris and uninformative cell types, former limitations to flow analysis of sputum specimens. The system, comprised of the specimen processor and the FOT analysis instrument, is expected to have applicability in high throughput testing environments.

描述（由申请人提供）：流动光学断层扫描（FOT）是一种新技术，目前处于Vision Gate Inc.的早期开发阶段，用于快速成像高通量流动流中的单个染色细胞。多个点投影源和检测器生成2D和3D密度图像和亚细胞分辨率的色团定量。在这项试点研究中，我们以过去的经验为基础，与Vision Gate合作，通过三个目标评估FOT的潜力：首先，我们将从市售核染料中选择与FOT技术最兼容的核染料。其次，我们将利用现有的成像技术，从核染色和免疫细胞化学标记物中创建和验证癌前病变相关特征的数字库。最后，我们将确定FOT是否可以从正在进行的Moffitt肺癌筛查研究中具有已知临床结果的个体产生的存档痰标本的高通量流中捕获肺癌和癌前细胞的这些2D形态和密度特征。在拟议的研究中，我们将评估FOT以高通量方式捕获的能力，使用形态学和密度测定法，为肺癌早期检测提供相关诊断信息，从而能够开发大规模筛查平台。Vision Gate技术还通过在一项单独的研究中开发一种高吞吐量的生物处理设备来支持该项目。这为FOT提供了标本，将通过去除混杂碎片和无信息细胞类型（痰液标本流动分析的前限制）进行富集。该系统由样品处理器和FOT分析仪器组成，预计将在高通量测试环境中具有适用性。