Infrared Laser Desorption Ion Mobility Mass Spectrometer

红外激光解吸离子淌度质谱仪

• 批准号: 6836137
• 负责人: MICHAEL UGAROV
• 金额: $ 9.99万
• 依托单位: IONWERKS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-29 至 2005-03-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6836137
• 关键词: aerosols; bioengineering /biomedical engineering; biomedical equipment development; chemical structure; clinical biomedical equipment; infrared spectrometry; intermolecular interaction; ionization; mass spectrometry; molecular size; structural biology

DESCRIPTION (provided by applicant): The goal of this research program is to develop an infrared laser desorption ionization (IR-LDI ion mobility mass spectrometer (IMMS) for application to the analysis of biomolecules in complex systems such as direct tissue studies and the identification of the components of bioaerosols. The IMMS instrument separates biomolecule ions in two dimensions. The first dimension is the gas phase mobility separation in which the ions resulting from laser desorption drift through a gas collision cell under the influence of a constant electric field and are separated due to differences in collision cross-section. After exiting the drift cell, the ions are orthogonally extracted into a time-of-flight mass spectrometer. Because the separation takes place in the gas phase, it is significantly more rapid than liquid phase separations such as liquid chromatography or capillary electrophoresis. The performance goals for the IR-LDI IMMS are an ion mobility resolution of 50, mass resolution of 3000, mass range or 20 kDa, and a detection limit of 10 fmol. The instrument will be constructed at Ionwerks in Houston, TX. Ionwerks was founded in 1986 and is an accomplished provider of mass spectrometer systems and equipment. As part of this research, Ionwerks is collaborating with the mass spectrometry research group at Louisiana State University who are experts in the use of IR lasers for the direct analysis of biomolecules. The group will develop IMMS methods for the rapid analysis of cells and tissue with minimal sample pre-treatment. Commercial applications of this technology are in biomedical research, clinical diagnostics, and the detection of biological agents.

描述（由申请人提供）：本研究计划的目标是开发一种红外激光解吸电离（IR-LDI）离子迁移质谱仪（IMMS），用于分析复杂系统中的生物分子，如直接组织研究和生物气溶胶组分的鉴定。IMMS仪器在两个维度上分离生物分子离子。第一维是气相迁移率分离，其中由激光解吸产生的离子在恒定电场的影响下漂移通过气体碰撞池，并且由于碰撞横截面的差异而分离。在离开漂移池之后，离子被正交提取到飞行时间质谱仪中。因为分离发生在气相中，所以它明显比液相分离（如液相色谱或毛细管电泳）更快。IR-LDI IMMS的性能目标是离子迁移率分辨率为50，质量分辨率为3000，质量范围为20 kDa，检测限为10 fmol。该仪器将在德克萨斯州休斯顿的Ionwerks建造。Ionwerks成立于1986年，是质谱仪系统和设备的成功供应商。作为这项研究的一部分，Ionwerks正在与路易斯安那州立大学的质谱研究小组合作，他们是使用红外激光直接分析生物分子的专家。该小组将开发IMMS方法，用于快速分析细胞和组织，只需最少的样品预处理。该技术的商业应用包括生物医学研究、临床诊断和生物制剂检测。

项目成果

IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry.

IR-MALDI-LDI 与离子淌度正交飞行时间质谱联用。

• DOI: 10.1021/pr060055l
• 发表时间: 2006
• 期刊: Journal of proteome research
• 影响因子: 4.4
• 作者: Woods,AminaS;Ugarov,Michael;Jackson,ShelleyN;Egan,Thomas;Wang,Hay-YanJ;Murray,KermitK;Schultz,JAlbert
• 通讯作者: Schultz,JAlbert