Cloning of 400 full-length large cDNAs

克隆 400 个全长大 cDNA

• 批准号: 6832889
• 负责人: ZAIREN SUN
• 金额: $ 21.79万
• 依托单位: ORIGENE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-17 至 2005-03-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6832889
• 关键词: biotechnology; complementary DNA; functional /structural genomics; genetic library; genetic transcription; high throughput technology; human genetic material tag; messenger RNA; method development; microarray technology; molecular biology information system; molecular cloning; nucleic acid hybridization; nucleic acid probes; nucleic acid purification; nucleic acid quantitation /detection; nucleic acid sequence; open reading frames; proteomics

DESCRIPTION (provided by applicant): The latest build of human genome "build 34" contains 21,335 unique reference sequences that represent 17,869 unique loci. A survey of the open reading frame (ORF) size reveals that 635 reference sequences contain ORFs greater than 5 kb, but only 11 of those larger clones are commercially available through the Mammalian Gene Collection (MGC). The availability of a physical clone of the gene of interest is absolutely essential for functional analyses. OriGene Technologies, Inc. has developed 20 state-of-the-art cDNA libraries, the Rapid-Screen Arrayed cDNA library Panels, to support high-throughput full-length cDNA cloning. To date, we have also isolated and sequenced 800,000 individual clones from our primary cDNA libraries (non-arrayed) and have released 20,000 unique full-length genes, the largest in the industry, for functional genomics research. Phase I of this project aims to optimize experiments in order to obtain 400 of those very large transcripts, including the identification of partial, overlapping clones associated with each gene. This will require the optimization of primer design for cloning using our Rapid-Screen technology. Phase II of the project will focus on the cloning and complete sequencing of 400 of those long clones to make them available to the research community. We believe that our technology platform is reliable and innovative, and the outcome of this project will be assuredly a commercial success.

描述（由申请人提供）：最新构建的人类基因组“构建34”包含代表17，869个独特基因座的21，335个独特参考序列。对开放阅读框（ORF）大小的调查显示，635个参考序列含有大于5 kb的ORF，但这些较大的克隆中只有11个可通过哺乳动物基因保藏中心（MGC）商购获得。目的基因的物理克隆的可用性对于功能分析是绝对必要的。OriGene Technologies，Inc.已经开发了20个最先进的cDNA文库，快速筛选阵列cDNA文库面板，以支持高通量全长cDNA克隆。到目前为止，我们还从我们的主要cDNA文库（非阵列）中分离和测序了800，000个单独的克隆，并发布了20，000个独特的全长基因，这是业界最大的，用于功能基因组学研究。该项目的第一阶段旨在优化实验，以获得400个非常大的转录本，包括鉴定与每个基因相关的部分重叠克隆。这将需要使用我们的快速筛选技术优化克隆引物设计。该项目的第二阶段将侧重于克隆和完成400个长克隆的测序，以提供给研究界。我们相信我们的技术平台是可靠且创新的，该项目的结果肯定会取得商业成功。