Tunable Source for Protein Fluorescence Lifetime Studies

用于蛋白质荧光寿命研究的可调源

• 批准号: 6835309
• 负责人: Gregory David Gillispie
• 金额: $ 18.71万
• 依托单位: DAKOTA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6835309
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; data collection methodology /evaluation; fluorescence; fluorescence spectrometry; method development; nanotechnology; protein quantitation /detection

DESCRIPTION (provided by applicant): Dakota Technologies, Inc. (DTI) has developed and demonstrated a low-cost and user-friendly method that generates fluorescence decay curves with exceptional speed, accuracy, and precision. Sample fluorescence is induced by a Nd:YAG microchip laser that operates at high repetition rate (5-10 kHz) and delivers sub-nanosecond pulses. Waveforms of the resulting fluorescence decay are directly recorded via a photomultiplier tube (PMT) and fast digitizer. The available excitation wavelengths are limited to the Nd:YAG harmonics at 532, 355, and 266 nm. Excitation sources that can be continuously tuned over a broad wavelength range and retain the high pulse repetition frequency and short pulse duration will be developed during this Phase I SBIR project. Particular emphasis will be placed on the 275-315 nm range that is optimal for fluorescence characterization of proteins. By perfecting these sources and integrating them into a commercial spectrofluorimeter, DTI will provide biochemists, biophysicists, and molecular biologists with a versatile, high performance tool for cutting edge research. A promising industrial application, verifying that proteins are produced in the appropriate conformation, has also been identified.

描述（由申请人提供）：Dakota Technologies，Inc. (DTI)开发并演示了一种低成本且用户友好的方法，该方法以出色的速度、准确性和精确度生成荧光衰减曲线。样品荧光由Nd：YAG微芯片激光器诱导，该激光器以高重复率（5-10 kHz）运行并提供亚纳秒脉冲。通过光电倍增管（PMT）和快速数字转换器直接记录所产生的荧光衰减的波形。可用的激发波长限于532、355和266 nm的Nd：YAG谐波。在第一阶段SBIR项目期间，将开发可以在宽波长范围内连续调谐并保持高脉冲重复频率和短脉冲持续时间的激发源。将特别强调275-315 nm范围，这是最佳的蛋白质的荧光表征。通过完善这些光源并将其集成到商业荧光分光光度计中，DTI将为生物化学家、生物化学家和分子生物学家提供一种用于尖端研究的多功能、高性能工具。一个有前途的工业应用，验证蛋白质产生的适当的构象，也已确定。