New Experimental and Computational Tools for Tissue Engineering

组织工程的新实验和计算工具

• 批准号: 7326746
• 负责人: Gregory David Gillispie
• 金额: $ 20.3万
• 依托单位: FLUORESCENCE INNOVATIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7326746
• 关键词: Affect; Biocompatible Materials; Biological Models; Biological Sciences; Biomedical Engineering; Cartilage; Cells; Cellular Structures; Chondrocytes; Clinical; Code; Collaborations; Collagen; Color; Computer Simulation; Computing Methodologies; Data; Databases; Dentistry; Deposition; Diagnostic; Dyes; Engineering; Equipment; Extracellular Matrix; Fluorescence; Fluorescence Spectroscopy; Goals; Green Fluorescent Proteins; Growth; Hand; Histology; Implant; Invasive; Knowledge; Label; Laboratories; Lead; Light; Liquid substance; Measurement; Measures; Medical; Methods; Michigan; Modeling; Molecular; Molecular Biology; Monitor; Morphologic artifacts; Natural regeneration; Normal tissue morphology; Numbers; Optics; Personal Satisfaction; Phase; Process; Prosthesis; Proteins; Research; Research Personnel; Sampling; Scientist; Signal Transduction; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solutions; Spatial Distribution; Specificity; Speed; Staining method; Stains; System; Techniques; Technology; Testing; Time; Tissue Engineering; Tissues; Universities; Validation; Week; Work; Wound Healing; analytical tool; articular cartilage; biodegradable polymer; college; computerized tools; crosslink; design and construction; desire; experience; fluorophore; implantation; improved; innovation; instrumentation; quantum; scaffold; soft tissue; tissue phantom

DESCRIPTION (provided by applicant): Tissue engineering aims to create functional biologic prostheses by suspending dissociated cells into a biodegradable polymer scaffold upon which new tissue forms. Better means are urgently needed to compare engineered tissues, particularly cartilage and other soft tissues, with normal tissue. Histology is slow, cumbersome, and destructive because it requires first sectioning the tissue, then staining it. In addition, the quantitative reliability of histology is marginal owing to high variability of the stain intensity and color. This Phase I SBIR application aims for a quantum leap forward in tissue assessment, which will be realized by combining a unique implementation of time-resolved fluorescence spectroscopy with Monte-Carlo code that models light propagation in tissue. Using articular cartilage constructs as a model system, our team will demonstrate quantitative tracking of collagen expression in the chondrocytes, chondrocyte proliferation, and the spatial distribution of the collagen cross-links forming adjacent to the chondrocytes in the extracellular matrix over the entire growth process. Specific Aim 1 is to track the activity of GFP that serves as a marker of collagen synthesis in chondrocytes and Specific Aim 2 is to track the activity of collagen cross-linker formation as it develops in the region around the chondrocytes. Once successfully demonstrated, these capabilities will make it possible to test a vastly expanded number of variables that potentially affect tissue growth and the ultimate function of the construct. Tests of high throughput tissue engineering in Phase II could ultimately lead to prostheses that perform better and for a longer period after implantation. The data and scientific knowledge gained during the Phase I effort also has potential applications in real-time medical diagnostics and wound healing. Tissue engineering creates functional biologic prostheses by growing cells that form new tissue on a biodegradable polymer scaffold. The goal of this Phase I SBIR application is improved techniques to test the laboratory grown tissues before they are implanted, resulting in prostheses that perform better and last longer.

描述（由申请人提供）：组织工程旨在通过将游离细胞悬浮到可生物降解的聚合物支架中来创建功能性生物假体，在该支架上形成新的组织。迫切需要更好的方法来比较工程组织，特别是软骨和其他软组织与正常组织。组织学是缓慢、繁琐和破坏性的，因为它需要先对组织切片，然后染色。此外，由于染色强度和颜色的高度可变性，组织学的定量可靠性是边缘的。这个第一阶段的SBIR应用旨在实现组织评估的量子飞跃，这将通过将时间分辨荧光光谱的独特实现与模拟光在组织中的传播的蒙特卡罗代码相结合来实现。利用关节软骨构建体作为模型系统，我们的团队将展示在整个生长过程中软骨细胞中胶原表达、软骨细胞增殖以及细胞外基质中胶原交联在软骨细胞附近形成的空间分布的定量跟踪。特异性目标1是追踪软骨细胞中胶原合成标记物GFP的活性，特异性目标2是追踪软骨细胞周围区域胶原交联剂形成的活性。一旦成功演示，这些能力将使测试大量可能影响组织生长和结构最终功能的变量成为可能。第二阶段的高通量组织工程测试可能最终导致假体在植入后表现更好，使用时间更长。在第一阶段工作中获得的数据和科学知识在实时医疗诊断和伤口愈合方面也有潜在的应用。组织工程通过在可生物降解的聚合物支架上培养细胞形成新组织来创造功能性生物假体。第一阶段SBIR应用的目标是改进技术，在植入之前对实验室培养的组织进行测试，从而使假体性能更好，使用寿命更长。