TWO DIMENSIONAL MASS SPECTROMETER FOR PROTEOMIC RESEARCH

描述（由申请人提供）：本项目的目标是开发一种串联二维质谱仪，该质谱仪将获得完整的MS/MS信息，包括来自所有前体及其母-子连接的碎片离子。来自离子源的离子将不会被过滤掉或有区别地选择，而是将它们中的每一个碎片化以获得MS/MS光谱。每个检测到的离子的强度将存储在二维阵列中，该阵列协调前体和产物离子的m/z比。二维质谱框架的扫描速度将相当于来自传统四极杆或离子阱仪器的全扫描速度，因此它将与LC-2DMS方法兼容。该仪器的开发将为蛋白质组学研究提供一个强有力的分析工具。对于高度复杂的蛋白质样品，它可以以更高的通量提供更好的肽覆盖率，具有更低的检测限，特别是对于低丰度蛋白质。通常来自不同MS/MS扫描模式的信息可以从单个2DMS扫描中提取。这将简化一些实验，并使该仪器成为一个多功能的蛋白质组学工作站。虽然这一拟议文书的概念采用了独特的混合组合，但这一文书所采用的大多数技术都是为现有文书开发的。因此，该提案失败的风险非常小，并且该技术所产生的好处将具有重要的商业应用。

DESCRIPTION (provided by applicant): The objective for this project is to develop a tandem two-dimensional mass spectrometer that will obtain complete MS/MS information, include fragmentation ions from all precursors with their parent-daughter linkage. The ions from the ion source will not be filtered out or discriminately selected, instead each of them will be fragmented to get MS/MS spectra. The intensities of each detected ion will be stored in a two dimensional array, which coordinates m/z ratios of precursor and product ions. The scan speed of a two dimensional mass spectra frame will be equivalent to the full scan speed from a traditional quadrupole or ion trap instrument, so it will compatible for LC-2DMS methods. Development of the proposed instrument would offer a powerful analytical tool for proteomics research. For highly complex protein samples, it could provide much better peptide coverage at higher throughput, with lower detection limits, especially for low abundant proteins. Information normally from different MS/MS scan modes can be extracted from a single 2DMS scan. This would simplify some experiments, and make this instrument a versatile proteomics workstation. Although the concept of this proposed instrument uses a unique hybrid combination, most technologies applied for this instrument were developed for existing instruments. Thus, the risk of failure for this proposal is very small and the resulting benefits of this technology would have significant commercial applications.