Micro-Fabricated Complete Blood Counter

微型全血计数器

• 批准号: 6788418
• 负责人: Harold Edward Ayliffe
• 金额: $ 10万
• 依托单位: E.I. SPECTRA, LLC
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2004-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6788418
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; blood cell count; consumable /disposable biomedical equipment; electrical impedance; erythrocytes; fluid flow; hematology; human tissue; leukocytes; miniature biomedical equipment; monitoring device; platelets; portable biomedical equipment; radiowave radiation

DESCRIPTION (provided by applicant): This SBIR research effort is aimed at the development and testing of a novel system for blood analysis that utilizes MEMS fabricated chips and multiple, radio-frequency electric impedance interrogation signals to differentiate cell types. Our long-term goals for the project can be summarized as three: 1) develop an inexpensive CBC analysis system utilizing disposable MEMS-based micro-fluidic chips for physician's offices, 2) miniaturize the electronic hardware and develop a highly portable CBC analyzer utilizing the same core technologies and 3) expand on the basic technologies (by integrating additional cell detection methods) to enhance system capabilities, performance and reliability. As part of the proposed long-term effort, we will fabricate, characterize and commercialize the first generation MEMS mu-CBC Analyzer. To achieve this, the present SBIR proposal addresses miniaturization of the conductance and impedance base cell counting techniques by incorporating disposable, micro-fabricated detectors into relatively inexpensive bench-top analysis systems. The first of three aims will concentrate on designing and micro-fabricating the next generation MEMS flow-chips suitable for proof-of-concept cell counting studies using radio-frequency impedance measurements. The second aim will target the development for the micro-fluidics required to drive the blood through the micro-channel at a flow-rate suitable for single file interrogation of cells. Aim three will focus on additional proof-of-concept studies to demonstrate feasibility of this technology to function as a hematology analyzer. Future work will build upon the results of the present proposal to create a field portable mu-CBC Analyzer based on the same micro-fluidic cell counting chip. We envision these mu-El systems will eventually lead to the development and commercialization of highly portable systems capable of drastically improving the ease (and availability) of administering blood tests (4-6 years). For example, these systems will enable field tests for remote patients with HIV, rapid testing for incoming emergency room patients, and self-administered home blood testing. These future multiple-detector systems will become extremely cost and performance competitive with the state-of-the-art hematology analyzers (6-10 years). To our knowledge, there are no other commercial efforts to develop blood cell counting instrumentation using MEMS-based technology.

描述（由申请人提供）：这项SBIR研究工作旨在开发和测试一种新型血液分析系统，该系统利用MEMS制造的芯片和多个射频电阻抗询问信号来区分细胞类型。我们对该项目的长期目标可以概括为三个：1）开发一种廉价的CBC分析系统，该系统利用基于MEMS的一次性微流控芯片，用于医生办公室，2）简化电子硬件，并开发一种高度便携的CBC分析仪，该分析仪利用相同的核心技术，3）扩展基本技术（通过集成附加的小区检测方法）来增强系统能力、性能和可靠性。作为拟议长期努力的一部分，我们将制造、表征和商业化第一代MEMS mu-CBC分析仪。为了实现这一点，本SBIR建议解决的电导和阻抗的基础细胞计数技术的小型化，将一次性的，微制造的检测器到相对便宜的台式分析系统。三个目标中的第一个将集中于设计和微制造下一代MEMS流式芯片，适用于使用射频阻抗测量的概念验证细胞计数研究。第二个目标将针对微流体技术的发展，所述微流体技术需要以适合于细胞的单列询问的流速驱动血液通过微通道。目标三将侧重于额外的概念验证研究，以证明该技术作为血液分析仪的可行性。未来的工作将建立在本建议的结果，以创建一个现场便携式mu-CBC分析仪基于相同的微流控细胞计数芯片。我们设想这些mu-El系统将最终导致高度便携式系统的开发和商业化，该系统能够大幅提高血液检测的易用性（和可用性）（4-6年）。例如，这些系统将能够对远程艾滋病毒患者进行现场检测，对急诊室患者进行快速检测，以及自行进行家庭血液检测。这些未来的多检测器系统将在成本和性能方面与最先进的血液分析仪竞争（6-10年）。据我们所知，没有其他商业努力来开发使用基于MEMS的技术的血细胞计数仪器。