Developing enhanced production and separation techniques for glycolipid biosurfactants

点击翻译按钮获取中文摘要

Glycolipid biosurfactants are naturally occurring amphiphiles with a range of unique properties including biodegradability and tolerance of a wide range of temperature and pH. These propertieshave facilitated the commercialisation of these biosurfactants by a number of companies in recent years, in a range of cleaning and personal care products.There is significant interest in molecules produced from industrial biotechnology routes and using biosurfactants such as sophorolipids as bulk chemicals, in particular for bioremediation andenhanced oil recovery, though the current cost of production is currently prohibitive. This is in part because of the requirement for feeding large quantities of substrate, meaning there is a requirementfor significant unused bioreactor volume at the beginning of the fermentation, and the high viscosity of the sophorolipid phase, which accumulates in the bioreactor over time inhibiting oxygen masstransfer to the cells.We have developed a novel gravity separation process1 to separate the sophorolipid product as it is produced and remove it from the bioreactor, reducing the bioreactor volume requirement andthereby enhancing productivity and fermentation product titre, and are working to exploit this technology commercially. These commercialisation activities have been recognised by a BBSRCInnovator of the Year Early Career Award.This PhD project will aim to further enhance fermentation and separation techniques for glycolipid biosurfactants, potentially facilitating further decreases in production costs. The project will build on current funded work with industrial collaborators to develop a controllable, scalable integrated bioprocess.1Dolman, BD et al. 2017, Process Biochemistry, 54(162-171)