Neural Gene Expression During Adaptive Plasticity

适应性可塑性期间的神经基因表达

基本信息

项目摘要

DESCRIPTION (provided by applicant): Experience shapes the functional capacity of the brain to suit the unique needs and environment of the individual. How such adaptive change occurs is of great interest as it circumscribes the individual's long-term behavior and capabilities. Despite this importance, the underlying molecular mechanisms are largely unknown. The goal of this proposal is to identify how the global pattern of neural gene expression changes during adaptive plasticity. The neural circuit under study is the barn owl sound localization pathway. Within this pathway, auditory and visual maps of space are aligned and integrated to yield a unified representation of the animal's surround. The co-registration of these maps can be manipulated by fitting owls with horizontally displacing prismatic spectacles (prisms). After several weeks of prism experience, the auditory space maps in the external nucleus of the inferior colliculus (ICX) and optic tectum (OT) adaptively adjust, restoring proper alignment and behavioral performance. This adaptive plasticity involves topographically appropriate axonal sprouting and synaptogenesis. The scope and persistence of structural remodeling strongly suggest that changes in neural gene expression underlie adaptive adjustment. To identify the crucial plasticity molecules, we will analyze genome-wide expression in the ICX and OT using serial analysis of gene expression (SAGE). The first set of experiments focuses on prism adaptation in juvenile owls. Functional phases of adaptive plasticity will be defined by auditory mapping experiments. SAGE analysis will be performed on adapted and non-adapted parts of the auditory map extracted from the same individual. Direct comparison will reveal the known and novel neural genes that are differentially regulated by prism experience. The second set of experiments will identify changes in gene expression associated with the enhanced capacity for plasticity displayed by prism-adapted adult owls. The third set of experiments involves mapping the spatio.-temporal patterns of expression of differentially regulated transcripts to different neuronal cell types using in situ hybridization. We expect that the data obtained by this approach will be broadly applicable to higher mammals including humans. Our long-term objective is to identify novel genes that play crucial roles during normal learning processes, and by extension, whose malfunction may contribute to learning disabilities and neurological disorders that involve a failure in neuroplasticity.
描述(由申请人提供):经验塑造大脑的功能能力,以适应个人的独特需求和环境。这种适应性变化是如何发生的是非常感兴趣的,因为它限制了个人的长期行为和能力。尽管如此重要,其潜在的分子机制在很大程度上是未知的。这个提议的目的是确定在适应性可塑性过程中神经基因表达的全球模式如何变化。所研究的神经回路是仓鸮声音定位通路。在这条通路中,听觉和视觉的空间地图被对齐并整合,以产生对动物周围环境的统一表示。这些地图的配准可以通过给猫头鹰安装水平位移棱镜眼镜(棱镜)来操作。经过几周的棱镜体验,下丘(ICX)和视顶盖(OT)的外部核中的听觉空间图自适应地调整,恢复正确的对齐和行为表现。这种适应性可塑性包括拓扑学上适当的轴突发芽和突触发生。结构重塑的范围和持久性强烈表明,神经基因表达的变化是适应性调整的基础。为了确定关键的可塑性分子,我们将使用基因表达系列分析(SAGE)分析ICX和OT中的全基因组表达。第一组实验的重点是青少年猫头鹰棱镜适应。适应性可塑性的功能阶段将通过听觉映射实验来定义。将对从同一个体提取的听觉图的适应和非适应部分进行SAGE分析。直接比较将揭示已知的和新的神经基因的差异调节棱镜的经验。第二组实验将确定与适应棱镜的成年猫头鹰表现出的可塑性增强能力相关的基因表达变化。第三组实验涉及绘制空间图。使用原位杂交对不同神经元细胞类型表达差异调节转录物的时间模式。我们期望通过这种方法获得的数据将广泛适用于包括人类在内的高等哺乳动物。我们的长期目标是确定在正常学习过程中发挥关键作用的新基因,并通过扩展,其功能障碍可能导致学习障碍和神经系统疾病,涉及神经可塑性的失败。

项目成果

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Wiliam McIntyre DeBello其他文献

Wiliam McIntyre DeBello的其他文献

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{{ truncateString('Wiliam McIntyre DeBello', 18)}}的其他基金

Integrative Analysis of Adaptive Information Processing and Learning-Dependent Circuit Reorganization in the Auditory System
听觉系统中自适应信息处理和学习依赖电路重组的综合分析
  • 批准号:
    10715925
  • 财政年份:
    2023
  • 资助金额:
    $ 29.7万
  • 项目类别:
From microscale structure to population coding of normal and learned behavior
从微观结构到正常和习得行为的群体编码
  • 批准号:
    10225540
  • 财政年份:
    2017
  • 资助金额:
    $ 29.7万
  • 项目类别:
From microscale structure to population coding of normal and learned behavior
从微观结构到正常和习得行为的群体编码
  • 批准号:
    9981045
  • 财政年份:
    2017
  • 资助金额:
    $ 29.7万
  • 项目类别:
From microscale structure to population coding of normal and learned behavior
从微观结构到正常和习得行为的群体编码
  • 批准号:
    9770569
  • 财政年份:
    2017
  • 资助金额:
    $ 29.7万
  • 项目类别:
Neural Gene Expression During Adaptive Plasticity
适应性可塑性期间的神经基因表达
  • 批准号:
    6534625
  • 财政年份:
    2002
  • 资助金额:
    $ 29.7万
  • 项目类别:
Neural Gene Expression During Adaptive Plasticity
适应性可塑性期间的神经基因表达
  • 批准号:
    6647750
  • 财政年份:
    2002
  • 资助金额:
    $ 29.7万
  • 项目类别:
The synaptic basis of learning in the auditory system
听觉系统学习的突触基础
  • 批准号:
    8076760
  • 财政年份:
    2002
  • 资助金额:
    $ 29.7万
  • 项目类别:
Neural Gene Expression During Adaptive Plasticity
适应性可塑性期间的神经基因表达
  • 批准号:
    7261343
  • 财政年份:
    2002
  • 资助金额:
    $ 29.7万
  • 项目类别:
The synaptic basis of learning in the auditory system
听觉系统学习的突触基础
  • 批准号:
    8463413
  • 财政年份:
    2002
  • 资助金额:
    $ 29.7万
  • 项目类别:
Neural Gene Expression During Adaptive Plasticity
适应性可塑性期间的神经基因表达
  • 批准号:
    7467376
  • 财政年份:
    2002
  • 资助金额:
    $ 29.7万
  • 项目类别:
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