Membrane properties of cells comprising the OHC system

Corti器官由多种细胞类型组成，包括感觉、支持和神经元件。综上所述，这些细胞组成了一个功能复杂且有凝聚力的电子单元，启动了对我们环境中的声学信息的分析。这个电子单元极其复杂，几乎无法在解剖学上接触到，这使得对整个系统的分析相当困难。幸运的是，在过去的几年里，体外方法，包括分离的耳蜗和细胞制剂，有助于阐明细胞的功能；策略是首先了解细胞的个体基础，最后将这些知识整合到对Corti器官的完整了解中。这个项目的总体目标是分析外毛细胞(OHC)的膜特性，外毛细胞是听觉功能的主要参与者之一，主要使用全细胞电压钳技术的变化。提出了三个方面的调查。具体地说，我们打算1)详细研究OHC侧膜与氯通量有关的机械敏感性，2)研究氯和阴离子对OHC马达活动的影响，3)研究Corti制剂外植体器官中毛细胞之间的机械耦合。我们将获得的结果将有助于更深入地了解内耳功能，并有助于理解听觉病理，这些病理可能是由于OHC侮辱和体内平衡失衡造成的，包括感音神经性听力损失和耳鸣。

The organ of Corti is composed of a variety of cell types including sensory, supporting and neural elements. Taken together, these cells comprise a functionally intricate and cohesive electrical unit that initiates the analysis of acoustic information within our environment. This electrical unit is extremely complex and nearly anatomically inaccessible, making an analysis of the whole quite a challenge. Fortunately, during the last several years the in vitro approach, including the isolated cochlea and cell preparations, has aided in the elucidation of cell function; the strategy is to understand the cells first on an individual basis, and finally to integrate this knowledge into a complete understanding of the organ of Corti. The overall aim of this project is to analyze the membrane properties of the outer hair cell (OHC), one of the major players in auditory function, principally using variations on the whole cell voltage clamp technique. Three areas of investigation are proposed. Specifically, we intend to 1) study in detail the mechanical sensitivity of the OHC lateral membrane as it relates to chloride flux, 2) study the effects of chloride and anions on the OHC motor activity, and 3) study mechanical coupling among OHCs in an explant organ of Corti preparation. The results that we will obtain will lead to a deeper understanding of inner ear function and aid in understanding auditory pathologies, which may result from OHC insult and homeostatic imbalance, including sensorineural hearing loss and tinnitus.